FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
par-6, l(2)gl
FlyBase ID
FBig0000039728
Interaction Network
Interactions Browser links
par-6 network
l(2)gl network
Reported Interactions
FBrf0157155-1.coIP.MS
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
l(2)gl 

Lgl

prey

46% coverage

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-11.PD.A
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

par-6 
par-6 
l(2)gl 

Lgl

prey

[35]S label

l(2)gl 
l(2)gl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
par-6 
PDZ domain
sufficient binding region
aa 146-280
par-6 
PDZ domain
necessary binding region
aa 147-258
l(2)gl 
N-terminal region
sufficient binding region
aa 1-217
l(2)gl 
central region
sufficient binding region
aa 402-802
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Betschinger et al., 2003)
FBrf0157155-4.coIP.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 

Lgl

bait
par-6 

Par-6

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-5.coIP.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Betschinger et al., 2003)
FBrf0157155-8.PD.WB
Reference
Betschinger et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 6embryonic stage 11embryonic stage 9early extended germ band stageembryonic stage 8embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Betschinger et al., 2003)
FBrf0179253-6.coIP.WB
Reference
Hutterer et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from transgenic construct; prey produced from endogenous gene.

(Hutterer et al., 2004)
FBrf0206088-10.PD.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GST tag

l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryo extract.

(Wirtz-Peitz et al., 2008)
FBrf0206088-2.coIP.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction of par-6 with l(2)gl is enhanced in aur mutants.

(Wirtz-Peitz et al., 2008)

Source was larval brains of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wirtz-Peitz et al., 2008)
FBrf0206088-6.coIP.WB
Reference
Wirtz-Peitz et al., 2008
Description
physical association
Assay
Collection
Source/Stage
larval brain / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait
l(2)gl 

Lgl

prey

Myc tag

l(2)gl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
l(2)gl 
aPKC phosphorylation sites
mutation disrupting interaction

\'3A\' construct, aPKC phosphorylation sites mutated to Ala

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval brains of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Wirtz-Peitz et al., 2008)
FBrf0217811-2.coIP.MS
Reference
Neumüller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
par-6 

Par-6

bait

GFP tag

l(2)gl 

Lgl

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged par-6 construct in a parδ226 mutant background.

(Neumüller et al., 2012)

Prey endogenous to embryonic extract.

(Neumüller et al., 2012)
FBrf0218395-624.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
l(2)gl 
unspecified role
l(2)gl 
unspecified role
l(2)gl 
unspecified role
par-6 
unspecified role
par-6 
unspecified role
par-6 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 230.74964

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)