WASp, Vrp1 Physical Interaction report

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Vrp1

Sltr

bait

FLAG tag

Vrp1

WASp

WASP

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Vrp1

C terminal region

sufficient binding region

This construct contains the WBD of Vrp1

Vrp1

C terminal region

necessary binding region

This construct has a deletion of the WBD of Vrp1

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim et al., 2007)

FBrf0202337-5.coIP.WB

Reference

Kim et al., 2007

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

WASP

bait

V5 tag

Vrp1

Sltr

prey

FLAG tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Kim et al., 2007)

FBrf0202337-6.PD.WB

Reference

Kim et al., 2007

Description

physical association

Assay

pull down

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Vrp1

Sltr

bait

GST tag

Vrp1

WASp

WASP

prey

V5 tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Vrp1

WBD

sufficient binding region

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced by recombinant protein transfected in S2R+ cell extract

(Kim et al., 2007)

FBrf0213682-3.coIP.WB

Reference

Jin et al., 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

WASP

bait

V5 tag

Vrp1

Sltr

prey

FLAG tag

Vrp1

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Vrp1

WASP-binding domain

necessary binding region

aa 683-706

deletion of an internal 24 amino acid sequence including the WASP-binding domain

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jin et al., 2011)

FBrf0213682-5.coIP.WB

Reference

Jin et al., 2011

Description

physical association

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

WASP

bait

FLAG tag

Vrp1

Sltr

prey

HA tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Jin et al., 2011)

WASp and blow bind competitively to Vrp1. Vrp1 binds to blow with 7-fold lower affinity than it binds WASp.

(Jin et al., 2011)

FBrf0213682-6.coIP.WB

Reference

Jin et al., 2011

Description

physical association

Assay

western blot

Collection

Source/Stage

Cell line used

experimental knowledge based

Participants

Corresponds to

Reported as

Role

Note

Vrp1

Sltr

bait

FLAG tag

WASp

WASP

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Jin et al., 2011)

WASp and blow bind competitively to Vrp1. Vrp1 binds to blow with 7-fold lower affinity than it binds WASp.

(Jin et al., 2011)

FBrf0218395-998.DPiM

Reference

Guruharsha et al., 2011

Description

physical association

Assay

Collection

DPiM-2

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

WASp

unspecified role

WASp

unspecified role

WASp

unspecified role

Vrp1

unspecified role

Vrp1

unspecified role

Vrp1

unspecified role

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

HGScore = 64.19756

FBrf0221091-4.coIP.WB

Reference

Kaipa et al., 2013

Description

physical association

Assay