FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cdk1, Cks30A
FlyBase ID
FBig0000059521
Interaction Network
Interactions Browser links
Cdk1 network
Cks30A network
Reported Interactions
FBrf0086403-1.Y2H
Reference
Finley et al., 1996
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 

DmCdc2

bait

fused to LexA DNA-binding domain

Cks30A 

Cdi2

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Finley et al., 1996)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Finley et al., 1996)
FBrf0187485-1.coIP.WB
Reference
Swan et al., 2005
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cks30A 

Cks30A

bait

Flag tag

Cks30A 
Cdk1 

Cdk1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cks30A 
interaction domain residue 61
mutation disrupting interaction
aa 61

P61L, coordinates relative to Cks30A-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Swan et al., 2005)

prey identified with antibody to Cdk1. Mutant described differently in figure and text, curated as described in text.

(Swan et al., 2005)
FBrf0215247-3.coIP.WB
Reference
Ghorbani et al., 2011
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 9blastoderm stagegastrula stageembryonic stage 8embryonic stage 6embryonic cycle 3embryonic stage 4embryonic cycle 9embryonic stage 2embryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cks30A 

Cks30A

bait

FLAG tag

Cdk1 

Cdk1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0218395-3749.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Cdk1 
unspecified role
Cdk1 
unspecified role
Cdk1 
unspecified role
Cks30A 
unspecified role
Cks30A 
unspecified role
Cks30A 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 274.31243

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)