γtub
Grip75
Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
γTub23C and Grip75 coimmunoprecipitate and cosediment as part of the γTuRC (γ-tubulin ring complex).
γ-tubulin
Source was embryos of wild-type fly line; proteins produced from endogenous gene.
γTub23C and Grip75 co-fractionate as part of a Sas-4-containing S-γ-TuRC (γ-tubulin ring) complex.
Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.