Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from ovary cDNA expression library).

Two-hybrid system: yeast LexA-BD/B42-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast LexA-BD/B42-AD

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

swa and sw bind the same groove in ctp, and are unlikely to bind ctp simultaneously.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

swa and ctp elute as a single peak on a gel filtration column.

Coexpression of ctp increases solubility and yield of swa protein.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

swa and ctp form a complex estimated to be 50kDa in size, in good agreement with the theoretical mass of 47.8kDa for the complex of dimeric ctp with dimeric swa.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

swa and ctp interact with Kd = 200nM.

Mutations that increase swa homodimerization also increase binding to ctp (Kd = 70nM). Similarly, mutations that reduce swa homodimerization reduce binding to ctp (500nM).

The authors suggests that ctp induces swa dimerization by binding the small proportion of dimeric swa in a population, and by mass action, shifting the swa population to the dimeric form.