FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
WASp, SH3PX1
FlyBase ID
FBig0000075154
Interaction Network
Interactions Browser links
WASp network
SH3PX1 network
Reported Interactions
FBrf0144932-12.coIP.WB
Reference
Worby et al., 2001
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
WASp 

Wasp

bait

V5 tag

SH3PX1 

DSH3PX1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Worby et al., 2001)
FBrf0144932-7.Y2H
Reference
Worby et al., 2001
Description
physical association
Assay
Collection
Source/Stage
organism / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
SH3PX1 

DSH3PX1

bait

fused to LexA DNA-binding domain

SH3PX1 
WASp 

Wasp

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SH3PX1 
SH3 domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/GAL4-AD

(Worby et al., 2001)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from larval cDNA expression library).

(Worby et al., 2001)
FBrf0147103-13.PD.WB
Reference
Worby et al., 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
WASp 

WASP

bait

V5 tag

SH3PX1 

DSH3PX1

prey

GST tag

SH3PX1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
SH3PX1 
phosphorylation site
mutation disrupting interaction
aa 56

Y56D, Y56E, but not Y56F

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified SH3PX1 was phosphorylated by Ack in vitro and tested for binding to WASp.

(Worby et al., 2002)

Phosphorylation of the Tyr-56 on SH3PX1, or mutation to a negatively charged residue, disrupts the interaction with WASp.

(Worby et al., 2002)

Interaction in vitro; bait derived from S2 cell extract; prey produced as a recombinant fusion protein in bacterial system.

(Worby et al., 2002)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)