FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
egl, ctp
FlyBase ID
FBig0000080354
Interaction Network
Interactions Browser links
egl network
ctp network
Reported Interactions
FBrf0174997-1.Y2H
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ctp 

Dlc

bait

fused to GAL4 DNA-binding domain

egl 

Egl

prey

fused to GAL4 activation domain

egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
C-terminal region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

(Navarro et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Navarro et al., 2004)
FBrf0174997-2.Y2H
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ctp 

Dlc

bait

fused to GAL4 DNA-binding domain

egl 

Egl

prey

fused to GAL4 activation domain

egl 
egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
C-terminal region
mutation decreasing interaction
aa 954

S954L, corresponds to egl[3e] allele

egl 
C-terminal region
mutation disrupting interaction
aa 954-958

S954L, S958R, \'dlc2pt\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Navarro et al., 2004)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Navarro et al., 2004)
FBrf0174997-3.coIP.WB
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

EE tag

egl 
egl 
egl 
ctp 

Dhc

prey

FLAG tag

ctp 
ctp 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
C-terminal region
sufficient binding region
aa 880-993
egl 
C-terminal region
mutation decreasing interaction
aa 954

S954L, corresponds to egl[3e] allele

egl 
C-terminal region
mutation disrupting interaction
aa 954-958

S954L, S958R, \'dlc2pt\'

ctp 
region
mutation disrupting interaction
aa 62

F62S, \'mutant 52\'

ctp 
region
mutation decreasing interaction
aa 73

F73S, \'mutant 74\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Navarro et al., 2004)
FBrf0174997-4.coIP.WB
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ctp 

Dhc

bait

FLAG tag

ctp 
ctp 
egl 

Egl

prey

EE tag

egl 
egl 
egl 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
C-terminal region
sufficient binding region
aa 880-993
egl 
C-terminal region
mutation decreasing interaction
aa 954

S954L, corresponds to egl[3e] allele

egl 
C-terminal region
mutation disrupting interaction
aa 954-958

S954L, S958R, \'dlc2pt\'

ctp 
region
mutation disrupting interaction
aa 62

F62S, \'mutant 52\'

ctp 
region
mutation decreasing interaction
aa 73

F73S, \'mutant 74\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of 293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Navarro et al., 2004)
FBrf0174997-5.coIP.WB
Reference
Navarro et al., 2004
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait
egl 
ctp 

Dhc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
C-terminal region
mutation decreasing interaction
aa 954

S954L, corresponds to egl[3e] allele

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovary of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Navarro et al., 2004)
FBrf0211628-6.coIP.WB
Reference
Bianco et al., 2010
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

ctp 

Dlc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Bianco et al., 2010)
FBrf0243139-2.coIP.WB
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

RFP tag

egl 
ctp 

Dlc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation decreasing interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2019)
FBrf0243139-5.coIP.WB
Reference
Goldman et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DRSC
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

RFP tag

egl 
ctp 

Dlc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
interaction domain residues 965,969
mutation disrupting interaction
aa 965,969

S965K,S969R, coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Goldman et al., 2019)
FBrf0250118-2a.coIP.WB
Reference
Neiswender et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

egl 
ctp 

Dlc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
egl 
2pt interaction domain residues 965 and 969
mutation decreasing interaction
aa 965 and 969

S965K and S969R; coordinates relative to egl-PB

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Endogenous egl was depleted with shRNA.

(Neiswender et al., 2021)

Source was ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Neiswender et al., 2021)
FBrf0251875-12.coIP.WB
Reference
Goldman et al., 2021
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
egl 

Egl

bait

GFP tag

ctp 

Dlc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult ovaries; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Goldman et al., 2021)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)