FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
rux, CycA
FlyBase ID
FBig0000082784
Interaction Network
Interactions Browser links
rux network
CycA network
Reported Interactions
FBrf0123013-1.coIP.WB
Reference
Foley et al., 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rux 

rux

bait
CycA 

CycA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from a heat shock transgenic construct; prey produced from endogenous gene.

(Foley et al., 1999)
FBrf0129710-1.coIP.WB
Reference
Avedisov et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
CycA 

CycA

bait
rux 

Rux

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Avedisov et al., 2000)
FBrf0129710-2.2H
Reference
Avedisov et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycA 

CycA

bait

fused to GAL4 DNA-binding domain

rux 

Rux

prey

fused to VP16 activation domain

rux 
rux 
rux 
rux 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
rux 
RTX binding motifs
sufficient binding region
aa 13-335aa 1-217

coordinates relative to rux-PA

coordinates relative to rux-PA

rux 
RTX binding motifs
necessary binding region
aa 1-33aa 196-333

coordinates relative to rux-PA

coordinates relative to rux-PA

rux 
RTX binding motif residue R248
mutation decreasing interaction
aa 248

R248A, coordinates relative to rux-PA

rux 
RTX binding motif residue L31
mutation disrupting interaction
aa 31

L31A, coordinates relative to rux-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was mammalian cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Avedisov et al., 2000)

Two-hybrid system: mammalian GAL4-BD/VP16-AD

(Avedisov et al., 2000)
FBrf0129710-3.2H
Reference
Avedisov et al., 2000
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rux 

Rux

bait

fused to GAL4 DNA-binding domain

CycA 

CycA

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was mammalian cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Avedisov et al., 2000)

Two-hybrid system: mammalian GAL4-BD/VP16-AD

(Avedisov et al., 2000)
FBrf0218395-1764.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CycA 
unspecified role
CycA 
unspecified role
CycA 
unspecified role
rux 
unspecified role
rux 
unspecified role
rux 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 190.01662

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)