Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

V12 form of Rac1 used to detect Rac1-slpr interaction.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

The Rac1-slpr interaction was stronger with the GTP bound form of Rac1.

Cloned reagents; interaction in solution.

Showed binding of Rac1-GTP at higher level than Rac1-GDP.