FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
stan, esn
FlyBase ID
FBig0000086821
Interaction Network
Interactions Browser links
stan network
esn network
Reported Interactions
FBrf0215818-1.Y2H
Reference
Matsubara et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
stan 

Fmi

bait
stan 
esn 

Esn

prey
esn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
stan 
C-terminal JM domain
unspecified role

\'juxtamembrane domain\'

esn 
LIM domain
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey was cloned reagent (previously isolated).

(Matsubara et al., 2011)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; low throughput.

(Matsubara et al., 2011)
FBrf0215818-2.coIP.WB
Reference
Matsubara et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
stan 

Fmi

bait

FLAG tag

stan 
esn 

Esn

prey

Myc tag

esn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
stan 
C-terminal cytoplasmic JM-A domain
unspecified role

\'juxtamembrane domain A\'

esn 
LIM domain
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction detected when polypeptides containing the stan JM domain (or JM-A subdomain) and the esn LIM domain expressed in S2 cells; no interaction detected when both full-length proteins expressed in S2 cells.

(Matsubara et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)