FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
CoRest, HDAC1
FlyBase ID
FBig0000091021
Interaction Network
Interactions Browser links
CoRest network
HDAC1 network
Reported Interactions
FBrf0180423-8.coIP.WB
Reference
Dallman et al., 2004
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
CoRest 

dCRL

bait

V5-tag

HDAC1 

Rpd3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cells; bait produced from transfected construct; prey produced from endogenous gene.

(Dallman et al., 2004)
FBrf0218395-3009.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 
unspecified role
HDAC1 
unspecified role
HDAC1 
unspecified role
CoRest 
unspecified role
CoRest 
unspecified role
CoRest 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 87.43187

FBrf0244272-15.coIP.WB
Reference
Mačinković et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-B2
Participants
Corresponds to
Reported as
Role
Note
CoRest 

dCoREST

bait

GFP tag

HDAC1 

dRPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from tagged endogenous gene; prey produced from endogenous gene.

(Mačinković et al., 2019)
FBrf0244272-17.coIP.MS
Reference
Mačinković et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-B2
Participants
Corresponds to
Reported as
Role
Note
CoRest 

dCoREST

bait

FLAG tag

HDAC1 

dRPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct or endogenous gene; prey produced from endogenous gene.

(Mačinković et al., 2019)
FBrf0244272-7.coIP.WB
Reference
Mačinković et al., 2019
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-B2
Participants
Corresponds to
Reported as
Role
Note
CoRest 

dCoREST

bait

FLAG tag

HDAC1 

dRPD3

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Mačinković et al., 2019)
FBrf0247928-1.TAP.MS
Reference
Takakura et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

Rpd3

bait

FLAG,HA tag

CoRest 

CoRest

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Takakura et al., 2021)
FBrf0247928-2.coIP.WB
Reference
Takakura et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

Rpd3

bait

HA tag

CoRest 

CoRest

prey

Myc tag

CoRest 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CoRest 
acetylation site residue 318
mutation decreasing interaction
aa 318

K318R mutation in CoRest-PC decreases interaction with HDAC1 yet has no effect in the context of CoRest-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene or tagged transgenic construct.

(Takakura et al., 2021)

The interaction between HDAC1 and the CoRest-PF isoform decreases upon neural activation while its interaction with the CoRest-PC isoform increases. These changes in binding are dependent on nej.

(Takakura et al., 2021)
FBrf0247928-3.PD.WB
Reference
Takakura et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
HDAC1 

Rpd3

bait

HA tag

CoRest 

CoRest

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction between HDAC1 and the CoRest-PC isoform increases by blocking HDAC1 deacetylase activity.

(Takakura et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in Sf9 cells; prey derived from transgenic adult head extract.

(Takakura et al., 2021)
FBrf0247928-4.PD.WB
Reference
Takakura et al., 2021
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CoRest 

CoRest

bait

Myc tag

HDAC1 

Rpd3

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The interaction between HDAC1 and the CoRest-PF isoform decreases upon neural activation.

(Takakura et al., 2021)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from transgenic adult head extract.

(Takakura et al., 2021)
FBrf0259942-2.BiFC.FM
Reference
Lv et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
CoRest 

CoRest

bait

mCerulean tag, N-terminal fragment

HDAC1 

HDAC1

prey

mCerulean tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2 cells; proteins produced from transfected constructs.

(Lv et al., 2024)

Figure incorrectly labeled. Interaction based on figure legend and text.

(Lv et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)