FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
skd, kto
FlyBase ID
FBig0000093164
Interaction Network
Interactions Browser links
skd network
kto network
Reported Interactions
FBrf0160638-1.coIP.WB
Reference
Janody et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
skd 

Skd

bait
kto 

Kto

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Janody et al., 2003)

extracts from transgenic line that over-expresses skd and kto

(Janody et al., 2003)
FBrf0160638-2.coIP.WB
Reference
Janody et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
skd 

Skd

bait
kto 

Kto

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Janody et al., 2003)
FBrf0160638-5.coIP.WB
Reference
Janody et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kto 

Kto

bait
skd 

Skd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts of transgenic fly line; bait produced from transgenic construct; prey produced from transgenic construct.

(Janody et al., 2003)

extracts from transgenic line that over-expresses skd and kto

(Janody et al., 2003)
FBrf0160638-6.coIP.WB
Reference
Janody et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kto 

Kto

bait
skd 

Skd

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts; bait produced from endogenous gene; prey produced from endogenous gene.

(Janody et al., 2003)
FBrf0218395-1641.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
kto 
unspecified role
kto 
unspecified role
kto 
unspecified role
skd 
unspecified role
skd 
unspecified role
skd 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 64.01463

FBrf0225834-5.coIP.WB
Reference
Mao et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
skd 

Skd

bait

Myc tag

kto 

Kto

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Cells were cotransfected with FLAG-ci, Myc-skd and HA-kto, then subjected to sequential two-step coimmunoprecipitation (anti-FLAG, then anti-Myc).

(Mao et al., 2014)

Sequential coIP indicates that skd and kto interact within a ci-containing complex.

(Mao et al., 2014)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Mao et al., 2014)
FBrf0226415-5.coIP.WB
Reference
Marr et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
skd 

skd

bait
kto 

kto

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Marr et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)