vkg and Col4a1 NC1 domains form a hexamer; multiple peptides identified after digestion by mass spectrometry from the NC1 domains of both vkg and Col4a1.

Source was extracts from DGRC Fly Pellet, stock 1661280.

vkg and Col4a1 NC1 expressed monomers form a trimer.

Interaction in vitro; proteins produced as recombinant fusion proteins in expiCHO-S system.

vkg and Col4a1 NC1 expressed monomers form a trimer.

Interaction in vitro; proteins produced as recombinant fusion proteins in expiCHO-S system.

Interaction in vitro; proteins produced as recombinant fusion proteins in expiCHO-S system.

CVC contains 1 vkg single chain monomer and 2 Col4a1 single chain monomers assembled into a single DNA construct with 7 aa linkers. vkg-Col4a1 formed a hexamer. Protein concentration, time, temperature, and divalent cation concentration accelerate the assembly of the hexamer.

Tissue extracted vkg and Col4a1 NC1 domains form a hexamer.

Source was extracts from DGRC Fly Pellet, stock 1661280.

Interaction in vitro; proteins produced as recombinant fusion proteins in expiCHO-S system.

CVC contains 1 vkg single chain monomer and 2 Col4a1 single chain monomers assembled into a single DNA construct with 7 aa linkers. vkg-Col4a1 formed a hexamer.