FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
sgg, Axn
FlyBase ID
FBig0000094868
Interaction Network
Interactions Browser links
sgg network
Axn network
Reported Interactions
FBrf0209417-2.Y2H
Reference
Kremer et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sgg 

Shaggy

bait

fused to GAL4 DNA-binding domain

Axn 

Axin

prey

fused to GAL4 activation domain

Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Axn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Axn 
Shaggy binding region residue L434
mutation decreasing interaction
aa 434

coordinates relative to Axn-PA

Axn 
Shaggy binding region residue E436
mutation decreasing interaction
aa 436

coordinates relative to Axn-PA

Axn 
Shaggy binding region residue E437
mutation decreasing interaction
aa 437

coordinates relative to Axn-PA

Axn 
Shaggy binding region residue E438
mutation disrupting interaction
aa 438

E438G, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue L439
mutation disrupting interaction
aa 439

L439P or L439Q, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue L443
mutation disrupting interaction
aa 443

L443R or L443Q, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue I444
mutation decreasing interaction
aa 444

coordinates relative to Axn-PA

Axn 
Shaggy binding region residue P445
mutation disrupting interaction
aa 445

P445R, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue K446
mutation disrupting interaction
aa 446

K446E or K446N, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue L447
mutation disrupting interaction
aa 447

L447S or L447Q, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue E449
mutation decreasing interaction
aa 449

coordinates relative to Axn-PA

Axn 
Shaggy binding region residue R452
mutation disrupting interaction
aa 452

K452P, coordinates relative to Axn-PA

Axn 
Shaggy binding region residue D455
mutation disrupting interaction
aa 455

D455G, coordinates relative to Axn-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Kremer et al., 2010)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Kremer et al., 2010)

Saturating mutagenesis of Axn prey to identify mutations that disrupt binding. Western blotting eliminated lost interaction due to nonsense mutations. Since clones contained, on average, 3 mutation each, single point mutations were subsequently tesetd.

(Kremer et al., 2010)
FBrf0217811-5.coIP.MS
Reference
Neumüller et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sgg 

Sgg

bait

YFP tag

Axn 

Axn

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a YFP-tagged sgg construct.

(Neumüller et al., 2012)

Prey endogenous to embryonic extract.

(Neumüller et al., 2012)
FBrf0242778-1.BiFC.FM
Reference
Lybrand et al., 2019
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
sgg 

Sgg

bait

YFP tag, Ven1 fragment

Axn 

Axin

prey

YFP tag, Ven2 fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Lybrand et al., 2019)

BiFC signal around nuclei was viewed only in regions of low wg signaling and may represent an active destruction complex.

(Lybrand et al., 2019)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)