Prey expressed and purified from bacteria.

Bait derived from in vitro translation.

GST pull down

Proteins endogenous to embryonic extract

RpS21 and sta proteins cosediment as part of native 40S ribosomal particle, but RpS21 is not found in polysomes. This interaction is disrupted by 500mM KCl.

Source was embryos of wild-type fly line; proteins produced from endogenous genes.

The structure of the Drosophila 80S ribosome with accessory proteins solved by cryo-EM. Complex captured as 3828 pairwise interactions which do not necessarily reflect direct contact.