FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
tacc, aurA
FlyBase ID
FBig0000094993
Interaction Network
Interactions Browser links
tacc network
aurA network
Reported Interactions
FBrf0144945-1.coIP.WB
Reference
Giet et al., 2002
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

Aurora A

bait
tacc 

D-TACC

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait endogenous to embryonic extract.

(Giet et al., 2002)

Prey endogenous to embryonic extract.

(Giet et al., 2002)
FBrf0144945-2.coIP.WB
Reference
Giet et al., 2002
Description
physical association
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tacc 

D-TACC

bait

GFP tag

aurA 

Aurora A

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a GFP-tagged tacc construct, P{Ubi-tacc.GFP}.

(Giet et al., 2002)

Prey endogenous to embryonic extract.

(Giet et al., 2002)
FBrf0144945-3.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
/ embryonic stage 1embryonic stage 12blastoderm stagegastrula stageembryonic stage 8embryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3embryonic stage 4embryonic stage 2embryonic cycle 9embryonic stage 5embryonic stage 11embryonic stage 3embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic cycle 4embryonic stage 10embryonic stage 7
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

AurA

enzyme

His tag

aurA 

AurA

enzyme

His tag

tacc 

TACC

enzyme target

GFP tag

tacc 

TACC

enzyme target

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0144945-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
aurA 

AurA

enzyme

His tag

aurA 

AurA

enzyme

His tag

tacc 

TACC

enzyme target

MBP tag

tacc 

TACC

enzyme target

MBP tag

tacc 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0233282-77.Y2H
Reference
Galletta et al., 2016
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
tacc 

TACC

bait

fused to GAL4 DNA-binding domain

tacc 
aurA 

Aurora A

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
tacc 
central region
sufficient binding region
aa 506-1063

coordinates relative to tacc-PC

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Galletta et al., 2016)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Galletta et al., 2016)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)