Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast LexA-BD/VP16-AD

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

CtBP binds h and @E(spl)mδ, but not other Hairy-class bHLH proteins.

Two-hybrid system: yeast LexA-BD/VP16-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

Weak binding.

h binds CtBP, but served as negative control for Rho1.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

cta binds both GDP and GTP bound Rho1.

Interaction unaffected by ubiquitylation of h protein.

Cloned reagents; interaction in solution.

Prey was cloned reagent (previously isolated).

Two-hybrid system: yeast LexA/VP16.