FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
snf, snRNA:U1:82Eb
FlyBase ID
FBig0000095232
Interaction Network
Interactions Browser links
snf network
snRNA:U1:82Eb network
Reported Interactions
FBrf0138511-4.coIP.NB
Reference
Nagengast and Salz, 2001
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

snf

bait
snRNA:U1:82Eb 

U1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait protein endogenous to extract.

(Nagengast and Salz, 2001)

Prey RNA endogenous to extract.

(Nagengast and Salz, 2001)
FBrf0211144-15.coIP.PCR
Reference
Gonsalvez et al., 2010
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

SNF

bait
snRNA:U1:82Eb 

U1snRNA

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gonsalvez et al., 2010)
FBrf0239188-2.XC
Reference
Weber et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

dSNF

neutral component
snf 

dSNF

neutral component
snf 
snf 

dSNF

neutral component
snf 
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
snf 
RRM domain
sufficient binding region
aa 1-96
snRNA:U1:82Eb 
SLI domain
sufficient binding region
GGCCGCAUUGCACCUCGCGGCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

(Weber et al., 2018)

The snf-U1 snRNA interaction was curated to the products of all 5 identical or nearly identical U1 snRNA genes.

(Weber et al., 2018)
FBrf0239188-21.ITC
Reference
Weber et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snRNA:U1:82Eb 

dU1

bait
snRNA:U1:82Eb 
snf 

dSNF

prey
snf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
snf 
RRM domain
sufficient binding region
aa 1-96
snRNA:U1:82Eb 
SLI domain
sufficient binding region
GGCCGCAUUGCACCUCGCGGCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

(Weber et al., 2018)

Kd = 34nM. The snf-U1 snRNA interaction is stronger when snf is prebound to U2A, lowering the Kd to 16.5nM. The snf-U1 snRNA interaction was curated to the products of all 5 identical or nearly identical U1 snRNA genes.

(Weber et al., 2018)
FBrf0239188-7.NMR
Reference
Weber et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
snf 

dSNF

neutral component
snf 

dSNF

neutral component
snf 
snf 

dSNF

neutral component
snf 
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 

dU1

neutral component
snRNA:U1:82Eb 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
snf 
RRM domain
sufficient binding region
aa 1-101
snRNA:U1:82Eb 
SLI domain
sufficient binding region
GGCCGCAUUGCACCUCGCGGCC
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

(Weber et al., 2018)

The snf-U1 snRNA interaction was curated to the products of all 5 identical or nearly identical U1 snRNA genes.

(Weber et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)