FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Axn, CAP
FlyBase ID
FBig0000095235
Interaction Network
Interactions Browser links
Axn network
CAP network
Reported Interactions
FBrf0152199-1.Y2H
Reference
Yamazaki and Nusse, 2002
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 17blastoderm stagegastrula stageembryonic stage 8dorsal closure stageembryonic stage 9embryonic stage 6early extended germ band stageembryonic cycle 3extended germ band stageembryonic stage 4embryonic cycle 9embryonic stage 2late extended germ band stageembryonic stage 5embryonic stage 11embryonic stage 3embryonic stage 12embryonic stage 15embryonic cycle 6embryonic cycle 5embryonic cycle 8embryonic cycle 7embryonic stage 13embryonic cycle 4embryonic stage 14embryonic stage 10embryonic stage 7embryonic stage 16
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Axn 

Axin

bait

fused to LexA DNA-binding domain

Axn 
CAP 

DCAP

prey

fused to activation domain

CAP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Axn 
region
unspecified role
aa 361-600aa 500-747
CAP 
SH3 domain
unspecified role
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA.

(Yamazaki and Nusse, 2002)

Prey derived from embryonic cDNA library.

(Yamazaki and Nusse, 2002)
FBrf0152199-2.PD.WB
Reference
Yamazaki and Nusse, 2002
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
CAP 

DCAP

bait

GST tag

Axn 

Axin

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in a baculovirus-Sf9 cell system.

(Yamazaki and Nusse, 2002)

Prey expressed in a baculovirus-Sf9 cell system.

(Yamazaki and Nusse, 2002)
FBrf0158734-6.Y2H
Reference
Yamazaki and Yanagawa, 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Axn 

Axin

bait

fused to LexA DNA-binding domain

CAP 

DCAPS

prey

fused to B42 activation domain

CAP 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CAP 
SH3 domains
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Yamazaki and Yanagawa, 2003)

Two-hybrid system: yeast LexA-BD/B42-AD

(Yamazaki and Yanagawa, 2003)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)