FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ntc, PI31
FlyBase ID
FBig0000095248
Interaction Network
Interactions Browser links
ntc network
PI31 network
Reported Interactions
FBrf0213568-1.coIP.MS
Reference
Bader et al., 2011
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ntc 

Nutcracker

bait

protein A tag

PI31 

DmPI31

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from transgenic line carrying a protein A-tagged ntc construct, P{dj-PrA-ntc}.

(Bader et al., 2011)

Prey endogenous to testis extract.

(Bader et al., 2011)
FBrf0213568-2.coIP.MS
Reference
Bader et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ntc 

Nutcracker

bait

protein A tag

PI31 

DmPI31

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from transfected cells.

(Bader et al., 2011)

Prey endogenous to cell extract.

(Bader et al., 2011)
FBrf0213568-4.coIP.WB
Reference
Bader et al., 2011
Description
physical association
Assay
Collection
Source/Stage
testis / adult stagemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ntc 

Nutcracker

bait

protein A tag

PI31 

PI31

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The ntc F box domain is dispensable for interaction with PI31.

(Bader et al., 2011)

Bait derived from transgenic line carrying a protein A-tagged ntc construct, P{dj-PrA-ntc}.

(Bader et al., 2011)

Prey endogenous to testis extract.

(Bader et al., 2011)
FBrf0213568-5.coIP.WB
Reference
Bader et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
ntc 

Nutcracker

bait

protein A tag

ntc 
PI31 

PI31

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
ntc 
V188
unspecified role
aa 188

necessary: V188E mutation weakens interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Bader et al., 2011)

Prey endogenous to cell extract.

(Bader et al., 2011)

Mutation of the conserved V188 residue in ntc weakens the interaction with PI31.

(Bader et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)