The C-terminal region of Psc (aa 697-1601) inhibits the interaction with Pc protein.

The C-terminal region of Pc (aa 1419-1589) inhibits the interaction with Psc protein.

Two-hybrid system: yeast LexA/B42.

The C-terminal region of Psc (aa 697-1601) inhibits the interaction with Pc protein.

The C-terminal region of Pc (aa 1419-1589) inhibits the interaction with Psc protein.! IN8b. Internal comments :Fig3D

Two-hybrid system: yeast LexA/B42.

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

GST pull down.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

ph-p, ph-d, Pc, Psc and Scm copurify as part of a polycomb repressive (PRC1) complex

Nuclear extract was fractionated on a Biorex 70 column, then used for FLAG-affinity purification of ph-p containing complexes.

Source was embryonic nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

ph-p, ph-d, Pc, Psc and Scm copurify as part of a large polycomb repressive (PRC1) complex, estimated to be 2-6 MDa in size (gel filtration).

Affinity purified ph-p containing complexes were fractionated by gel filtration to estimate complex size.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The antibodies used against ph-p are described in DeCamillis et al. Genes and Development vol. 6 1992.

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The antibodies used against ph-p are described in DeCamillis et al. Genes and Development vol. 6 1992.

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

The affinity purified ph-p-containing complex was subjected to additional gel filtration and heparin affinity fractionation.

ph-p, Pc, Psc and Sce form the PRC1 core complex.

Source was cell extract of baculovirus-infected Sf9 insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.

Source was cell extract of baculovirus-infected insect cell line; bait produced from transfected construct; prey produced from transfected construct.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

Positive control

Positive control.

Source was embryonic nuclear extract of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

The PRC1 complex consists of Psc, Pc and Sce.

Interaction in vitro; bait and prey produced recombinant fusion proteins in baculovirus and insect cell system.