FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Eb1, Kebab
FlyBase ID
FBig0000095272
Interaction Network
Interactions Browser links
Eb1 network
Kebab network
Reported Interactions
FBrf0215262-1.PD.A
Reference
Meireles et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

MBP tag

Kebab 

Kebab

prey

[35]S label

Kebab 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Kebab 
SxIP motif
unspecified role
aa 150aa 169

necessary: mutation greatly weakens interaction

necessary: mutation weakens interaction

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Meireles et al., 2011)

Prey derived from in vitro translation.

(Meireles et al., 2011)

The two SxIP motifs of Kebab contribute additively in the interaction with Eb1 and together contribute to most, if not all, of Kebab\'s interaction with Eb1.

(Meireles et al., 2011)

MBP pull down.

(Meireles et al., 2011)

Kebab was identified by screening pools of cDNAs for interaction with MBP-tagged Eb1; sub-pools were tested until a single responsible cDNA was identified.

(Meireles et al., 2011)
FBrf0215262-2.PD.WB
Reference
Meireles et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Eb1 

EB1

bait

MBP tag

Kebab 

Kebab

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Meireles et al., 2011)

Prey expressed in stably transfected cells.

(Meireles et al., 2011)

MBP pull down.

(Meireles et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)