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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095374
Interaction Network
Interactions Browser links
AP-1γ network
Afti network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0217589-1.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

dAP1-γ

bait

GST tag

Afti 

CG8538

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
γ-ear domain
unspecified role
aa 853-982
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

Prey expressed in transiently transfected cells.

Afti interacts specifically with the γ-ear domain of AP-1γ, not that of Gga.

FBrf0217589-2.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Afti 

CG8538

unspecified role
Afti 

CG8538

unspecified role
Afti 
Afti 

CG8538

unspecified role
Afti 

dγ-ear

unspecified role

dγ-ear

unspecified role

dγ-ear

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Afti 
γ-ear binding motifs
unspecified role
aa 301-451

necessary: deletion abolishes interaction

γ-ear domain
unspecified role
aa 853-982
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast.

Mutation of conserved residues of the AP-1γ ear domain abolishes the interaction with Afti protein.

Afti protein interacts specifically with the γ-ear domain of AP-1γ, not that of Gga.

FBrf0217589-6.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Afti 

dAfti

bait

V5 tag

γ-adaptin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cells.

Prey endogenous to cell extract.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)