FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Afti, AP-1γ
FlyBase ID
FBig0000095374
Interaction Network
Interactions Browser links
Afti network
AP-1γ network
Reported Interactions
FBrf0217589-1.PD.WB
Reference
Kametaka et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
AP-1γ 

dAP1-γ

bait

GST tag

AP-1γ 
Afti 

CG8538

prey

V5 tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
AP-1γ 
γ-ear domain
unspecified role
aa 853-982
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Kametaka et al., 2012)

Prey expressed in transiently transfected cells.

(Kametaka et al., 2012)

Afti interacts specifically with the γ-ear domain of AP-1γ, not that of Gga.

(Kametaka et al., 2012)
FBrf0217589-2.Y2H
Reference
Kametaka et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Afti 

CG8538

unspecified role
Afti 

CG8538

unspecified role
Afti 
Afti 

CG8538

unspecified role
Afti 
AP-1γ 

dγ-ear

unspecified role
AP-1γ 
AP-1γ 

dγ-ear

unspecified role
AP-1γ 

dγ-ear

unspecified role
AP-1γ 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Afti 
γ-ear binding motifs
unspecified role
aa 301-451

necessary: deletion abolishes interaction

AP-1γ 
γ-ear domain
unspecified role
aa 853-982
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast.

(Kametaka et al., 2012)

Mutation of conserved residues of the AP-1γ ear domain abolishes the interaction with Afti protein.

(Kametaka et al., 2012)

Afti protein interacts specifically with the γ-ear domain of AP-1γ, not that of Gga.

(Kametaka et al., 2012)
FBrf0217589-6.coIP.WB
Reference
Kametaka et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Afti 

dAfti

bait

V5 tag

AP-1γ 

γ-adaptin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in stably transfected cells.

(Kametaka et al., 2012)

Prey endogenous to cell extract.

(Kametaka et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)