FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
p120ctn, shg
FlyBase ID
FBig0000095487
Interaction Network
Interactions Browser links
p120ctn network
shg network
Reported Interactions
FBrf0155865-1.coIP.WB
Reference
Myster et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p120ctn 

p120

bait

Myc tag

shg 

DE-Cad

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait derived from a transgenic line carrying a Myc-tagged p120ctn construct, P{Ubi-p63E-p120ctn.myc}.

(Myster et al., 2003)

Prey endogenous to embryonic extract.

(Myster et al., 2003)
FBrf0155865-6.Y2H
Reference
Myster et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p120ctn 

p120

bait

fused to LexA DNA-binding domain

p120ctn 
shg 

DE-Cad

prey

fused to GAL4 activation domain

shg 
shg 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shg 
cytoplasmic domain
unspecified role
aa 1350–1391
shg 
JM region
unspecified role
aa 1373–1378

necessary: clustered alanine substitutions abolish interaction

p120ctn 
Arm repeats 1-10
unspecified role
aa 211-690
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA/GAL4-AD.

(Myster et al., 2003)

Removal of two N-terminal or two C-terminal Arm repeats from p120ctn abolishes its interaction with shg.

(Myster et al., 2003)
FBrf0200619-44.PD.A
Reference
Liu et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
shg 

cad

bait

GST tag

shg 
p120ctn 

p120-catenin

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
shg 
juxtamembrane domain
sufficient binding region
aa 1346-1390

construct "A", coordinates relative to shg-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Liu et al., 2008)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)