FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
numb, mira
FlyBase ID
FBig0000095518
Interaction Network
Interactions Browser links
numb network
mira network
Reported Interactions
FBrf0098349-3.PD.A
Reference
Shen et al., 1997
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Nb

bait

GST tag

mira 

Miranda

prey

[35]S label

mira 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mira 
central region
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Shen et al., 1997)
FBrf0103035-3.PD.A
Reference
Shen et al., 1998
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
numb 

Numb

bait

GST tag

numb 
mira 

mira

prey

[35]S label

mira 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mira 
region
unspecified role
aa 300-467
numb 
N-terminal region
unspecified role
aa 1-223
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

(Shen et al., 1998)

Prey derived from in vitro translation.

(Shen et al., 1998)

mira protein binds insc, pros and numb proteins through distinct domains.

(Shen et al., 1998)

GST pull down.

(Shen et al., 1998)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)