Bait protein endogenous to extract.

Prey RNA endogenous to extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey RNA chemically synthesized and labeled in vitro.

One of several identical U2 snRNA genes chosen by curator to represent interaction. Addition of U2A greatly increases affinity of snf to U2 snRNA stemloop IV.

Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA chemically synthesized.

The snf-U2 snRNA interaction was curated to the products of all 6 identical or nearly identical U2 snRNA genes.

Interaction in vitro; proteins produced as a recombinant fusion protein in bacterial system.

Kd = 782nM. The snf-U2 snRNA interaction is stronger when snf is prebound to U2A, lowering the Kd to 117nM. The snf-U2 snRNA interaction was curated to the products of all 6 identical or nearly identical U2 snRNA genes.