Cross-linking not detected in ovary extracts derived from an exu⁴ background.

Bait RNA derived from in vitro transcription.

Prey endogenous to ovary extract.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide chemically synthesized and labeled in vitro.

exu binds bcd hairpin Vb with Kd = 15nM. exu bound bcd antisense hairpin Vb oligo (that retained similar structure) and a (UG)10 ssRNA with similar affinity.