Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).
Weak interaction.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Bait endogenous to testis extract.
Prey endogenous to testis extract.
Source was testes of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.