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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000095657
Interaction Network
Interactions Browser links
Trc8 network
CSN5 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0206144-3.PD.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trc8 

DTrc8

bait

GST tag

Trc8 
CSN5 

JAB1

prey

[35]S label

CSN5 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trc8 
RING-H2 domain
sufficient binding region
aa 562-744
CSN5 
MPN motif
sufficient binding region
aa 55-187
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed and purified from bacteria.

Prey derived from in vitro translation.

GST pull down.

FBrf0206144-4.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trc8 

DTrc8

bait

fused to GAL4 DNA-binding domain

Trc8 
CSN5 

JAB1

prey

fused to GAL4 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trc8 
Patched-like loop and RING-H2 finger
sufficient binding region
aa 277-744

lacks C-terminal PEST sequences

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Prey isolated from embryonic cDNA library.

Two-hybrid system: yeast GAL4-BD/GAL4-AD.

FBrf0206286-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trc8 

DTrc8

bait

fused to GAL4 DNA-binding domain

Trc8 
CSN5 

CSN5

prey

fused to GAL4 activation domain

CSN5 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CSN5 
MPN domain
mutation decreasing interaction
aa 100

T100I

Trc8 
C-terminal domain
sufficient binding region
aa 277-744
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

FBrf0206286-2.PD.A
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trc8 

DTrc8

bait

GST tag

Trc8 
CSN5 

CSN5

prey

[35]S label

CSN5 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
CSN5 
MPN domain
mutation decreasing interaction
aa 100

T100I

Trc8 
C-terminal domain
sufficient binding region
aa 562-809
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

FBrf0209743-3.PD.A
Reference
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Trc8 

Trc8

bait

GST tag

Trc8 
CSN5 

Csn5

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Trc8 
interaction domain
sufficient binding region
aa 562-809

coordinates relative to Trc8-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)