Diap1, HtrA2 Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

Diap1, HtrA2

FlyBase ID

FBig0000095661

Interaction Network

Interactions Browser links

Diap1 network
HtrA2 network

Reported Interactions

FBrf0200687-1.PD.MW

Reference

Challa et al., 2007

Description

physical association

Assay

pull down

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

GST tag

HtrA2

Omi

prey

HtrA2

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

HtrA2

serine protease and PDZ domains

sufficient binding region

aa 79-422aa 92-422

Construct based on a cleavage site at A79

Construct based on a cleavage site at A92

HtrA2

IAP binding motif AIIP

mutation disrupting interaction

aa 79

A to G mutation of the AIIP motif.

HtrA2

IAP binding motif ASKM

mutation disrupting interaction

aa 92

A to G mutation of the ASKM motif.

HtrA2

IAP binding motif AIIP

necessary binding region

aa 79-82

HtrA2

IAP binding motif ASKM

necessary binding region

aa 92-96

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Challa et al., 2007)

FBrf0200687-2.PD.WB

Reference

Challa et al., 2007

Description

physical association

Assay

pull down

western blot

Collection

Source/Stage

organism / embryonic stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

GST tag

HtrA2

Omi

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Challa et al., 2007)

FBrf0205481-1.PD.A

Reference

Khan et al., 2008

Description

physical association

Assay

pull down

autoradiography

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

HtrA2

dOmi

bait

His tag

HtrA2

Diap1

DIAP1

prey

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

HtrA2

IBM motif

mutation disrupting interaction

aa 75

A75P mutation abolishes interaction

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed and purified from bacteria.

(Khan et al., 2008)

Prey derived from in vitro translation.

(Khan et al., 2008)

th binds both large and small processed isoforms of HtrA2.

(Khan et al., 2008)

th binds a proteolytically inactive form of HtrA2 (S266A mutation).

(Khan et al., 2008)

A fragment of th remains associated with HtrA2 after cleavage of th by HtrA2.

(Khan et al., 2008)

His pull down.

(Khan et al., 2008)

FBrf0205481-2.ENZ.A

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

HtrA2

dOmi

enzyme

His tag

HtrA2

dOmi

enzyme

His tag

HtrA2

Diap1

DIAP1

enzyme target

[35]S label

Diap1

DIAP1

enzyme target

[35]S label

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0205481-7.ENZ.MW

Reference

Description

Assay

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

HtrA2

dOmi

enzyme

His tag

HtrA2

dOmi

enzyme

His tag

Diap1

DIAP1

enzyme target

GST tag

Diap1

DIAP1

enzyme target

GST tag

Diap1

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

FBrf0205481-8.PD.MW

Reference

Khan et al., 2008

Description

physical association

Assay

pull down

molecular weight estimation by staining

Collection

Source/Stage

Cell line used

Participants

Corresponds to

Reported as

Role

Note

Diap1

DIAP1

bait

GST tag

Diap1

HtrA2

dOmi

prey

His tag

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Diap1

BIR1

sufficient binding region

aa 1-165

Diap1

BIR2-RING

sufficient binding region

aa 166-438

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Bait expressed and purified from bacteria.

(Khan et al., 2008)

Prey expressed and purified from bacteria.

(Khan et al., 2008)

HtrA2 binds both BIR domains of th.

(Khan et al., 2008)

GST pull down.

(Khan et al., 2008)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

HtrA2,Diap1

References (2)

Report Sections

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