FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Sbf, Rab21
FlyBase ID
FBig0000095696
Interaction Network
Interactions Browser links
Sbf network
Rab21 network
Reported Interactions
FBrf0218866-10.PD.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait

FLAG tag

Sbf 

Sbf

prey

GFP tag

Sbf 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sbf 
DENN domain
sufficient binding region
aa 1-508
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified FLAG-tagged Rab21 was mixed with extracts from Kc167 cells expressing GFP-tagged Sbf.

(Jean et al., 2012)

Bait expressed and purified from bacteria.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)
FBrf0218866-11.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Sbf 

Sbf

bait

FLAG tag

Rab21 

Rab21

prey

GFP tag

Rab21 
Rab21 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rab21 
region
mutation increasing interaction
aa 27

T27N mutation mimics inactive GDP-bound state

Rab21 
region
mutation decreasing interaction
aa 73

Q73L mutation mimics active GDP-bound state

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)

Sbf interacts much more strongly with a dominant negative form of Rab21.

(Jean et al., 2012)
FBrf0218866-12.PD.A
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait

GST tag

Sbf 

Sbf

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sbf binds the GDP-bound form of Rab21, but not Rab21 bound to non-hydrolyzable GTPĪ³S

(Jean et al., 2012)

Bait expressed and purified from bacteria.

(Jean et al., 2012)

Prey derived from in vitro translation.

(Jean et al., 2012)
FBrf0218866-13.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sbf 

Sbf

enzyme
Sbf 

Sbf

enzyme
Sbf 
Rab21 

Rab21

enzyme target

FLAG tag

Rab21 

Rab21

enzyme target

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0218866-14.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sbf 

Sbf

enzyme
Sbf 

Sbf

enzyme
Sbf 
Rab21 

Rab21

enzyme target

FLAG tag

Rab21 

Rab21

enzyme target

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
FBrf0218866-15.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait

FLAG tag

Sbf 

Sbf

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)
FBrf0218866-9.coIP.WB
Reference
Jean et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Kc167
Participants
Corresponds to
Reported as
Role
Note
Sbf 

Sbf

bait

FLAG tag

Rab21 

Rab21

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sbf interacts strongly with Rab21 and weakly with Rab11, but not with Rab5.

(Jean et al., 2012)

Bait expressed in transfected cells.

(Jean et al., 2012)

Prey expressed in transfected cells.

(Jean et al., 2012)
FBrf0227704-1.coIP.WB
Reference
Jean et al., 2015
Description
physical association
Assay
Collection
Source/Stage
/ third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rab21 

Rab21

bait
Sbf 

Sbf

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was starved third instar larvae; bait produced from endogenous gene; prey produced from endogenous gene.

(Jean et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)