FB2026_02 , released June 18, 2026
FB2026_02 , released June 18, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Rel, cact
FlyBase ID
FBig0000095718
Interaction Network
Interactions Browser links
Rel network
cact network
Reported Interactions
FBrf0158946-7.Y2H
Reference
Minakhina et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rel 

Rel

bait

fused to LexA DNA-binding domain

cact 

Cactus

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast LexA-BD/B42-AD

(Minakhina et al., 2003)

Positive control.

(Minakhina et al., 2003)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Minakhina et al., 2003)
FBrf0218496-3.SPR
Reference
Bitra et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cact 

cactus

bait

His tag

cact 
Rel 

Relish

prey

Strep tag

Rel 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
Rel homology domain
sufficient binding region
aa 150-477
cact 
ankyrin repeat domain
sufficient binding region
aa 220-482
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Rel homodimers tested in this assay.

(Bitra et al., 2012)

Bait expressed and purified from bacteria.

(Bitra et al., 2012)

Prey expressed and purified from bacteria.

(Bitra et al., 2012)

cact binds the Rel homology domains of Dif and dl more strongly than that of Rel.

(Bitra et al., 2012)
FBrf0218496-9.PD.WB
Reference
Bitra et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Rel 

Relish

bait

Trx tag

Rel 
cact 

Cactus

prey

His tag

cact 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Rel 
Rel homology domain
sufficient binding region
aa 150-477
cact 
ankyrin repeat domain
sufficient binding region
aa 220-482
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Purified proteins were mixed and precipitated with anti-thioredoxin (Trx tag) antibodies.

(Bitra et al., 2012)

Bait expressed and purified from bacteria.

(Bitra et al., 2012)

Prey expressed and purified from bacteria.

(Bitra et al., 2012)

The cact ankyrin repeat domain binds the Rel homology domains of Dif and dl more strongly than the Rel Rel homology domain.

(Bitra et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)