FB2026_03 , released September 17, 2026
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
mib1, Ser
FlyBase ID
FBig0000095834
Interaction Network
Interactions Browser links
mib1 network
Ser network
Reported Interactions
FBrf0187469-3.coIP.WB
Reference
Lai et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mib1 

D-mib

bait

Myc tag

mib1 
Ser 

Serrate

prey

polyoma epitope tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mib1 
N-terminal region
sufficient binding region

\'D-mib-N\'; lacking RING domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK 293T (human) cell line transfected with tagged Ser and mib1 constructs.

(Lai et al., 2005)
FBrf0263772-1.coIP.WB
Reference
Seib et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
Ser 

Ser

bait

HA tag

mib1 

mib1

prey

V5 tag

mib1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mib1 
delta ring finger domain
sufficient binding region
unspecified
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

mib1 lacking the ring finger domain is able to interact with Ser and all of its variants.

(Seib et al., 2025)

Source was cell extract of S2R+ cell line; bait produced from transfected construct; prey produced from transfected construct.

(Seib et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)