FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
pan, Mad
FlyBase ID
FBig0000095977
Interaction Network
Interactions Browser links
pan network
Mad network
Reported Interactions
FBrf0206329-1.coIP.WB
Reference
Zeng et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pan 

dTCF

bait

MYC tag

Mad 

Mad

prey

T7 tag

Mad 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Mad 
Linker and MH2 domains
sufficient binding region
aa 157-455

coordinates relative to Mad-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zeng et al., 2008)

Mad and pan form a complex that competes with arm-pan binding. Mad and arm do not bind in this experiment.

(Zeng et al., 2008)
FBrf0206329-2.coIP.WB
Reference
Zeng et al., 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

T7 tag

pan 

dTCF

prey

MYC tag

pan 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
pan 
HMG domain
sufficient binding region
aa 1-394

coordinates relative to pan-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zeng et al., 2008)

Mad and pan form a complex that competes with arm-pan binding. Mad and arm do not bind in this experiment.

(Zeng et al., 2008)
FBrf0216411-2.coIP.WB
Reference
Eivers et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Mad 

Mad

bait

FLAG tag

pan 

Pan

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK293T cells.

(Eivers et al., 2011)

Prey expressed in transfected HEK293T cells.

(Eivers et al., 2011)
FBrf0216411-3.coIP.WB
Reference
Eivers et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
pan 

Pan

bait

Myc tag

Mad 

Mad

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected HEK293T cells.

(Eivers et al., 2011)

Prey expressed in transfected HEK293T cells.

(Eivers et al., 2011)

Activation of BMP signaling abolishes the interaction of Mad with pan, presumably due to phosphorylation of the Mad C-terminus.

(Eivers et al., 2011)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)