FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
mib1, Delta
FlyBase ID
FBig0000096189
Interaction Network
Interactions Browser links
mib1 network
Delta network
Reported Interactions
FBrf0187469-1.coIP.WB
Reference
Lai et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Delta 

Delta

bait

polyoma epitope tag

mib1 

D-mib

prey

Myc tag

mib1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mib1 
N-terminal region
sufficient binding region

\'D-mib-N\'; lacking RING domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK 293T (human) cell line transfected with tagged Dl and mib1 constructs.

(Lai et al., 2005)
FBrf0187469-2.coIP.WB
Reference
Lai et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mib1 

D-mib

bait

Myc tag

mib1 
Delta 

Delta

prey

polyoma epitope tag

Delta 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
mib1 
N-terminal region
sufficient binding region

\'D-mib-N\'; lacking RING domain

Delta 
intracellular domain
sufficient binding region

\'Dl[IC]\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was HEK 293T (human) cell line transfected with tagged Dl and mib1 constructs.

(Lai et al., 2005)
FBrf0216896-1.coIP.WB
Reference
Daskalaki et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Delta 

Dl

bait

V5 tag

Delta 
mib1 

Mib1

prey

Myc tag

mib1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Delta 
region
necessary binding region
aa 742-747

\'Dli2\'

mib1 
region
sufficient binding region

\'Mib1ΔR\'; lacks RING domain

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was S2 cell line transfected with tagged Dl and mib1 constructs.

(Daskalaki et al., 2011)

Assay for docking site of mib1 protein; enzymatic domain removed.

(Daskalaki et al., 2011)
FBrf0216896-4.coIP.ENZ
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
mib1 

Mib1

enzyme
mib1 

Mib1

enzyme
Delta 

Dl

enzyme target

His tag

Delta 

Dl

enzyme target

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)