Source was embryonic cDNA library.
Two-hybrid system: yeast LexA/VP16.
Cloned reagents; interaction in solution.
Prey was cloned reagent (previously isolated).
Two-hybrid system: yeast LexA/VP16.
Weak interactions observed with two smaller (non-overlapping) p53 C-terminal fragments, oligomerization domain C1 (amino acids 277-353) and basic region C2 (352-385).
K302R modification of aa 302 (within a canonical SUMO acceptor site) has no affect upon this interaction.
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pairwise.)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein.(Pooled)
MORATORIUM:This data was made available \'pre-publication\' and, as such, its use is subject to the specific limitations described in FBrf0235788 for the period of 12 months. Moratorium expires on June 13, 2018.
Two-hybrid system: yeast GAL4-BD/GAL4-AD
