FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
cuff, rhi
FlyBase ID
FBig0000096244
Interaction Network
Interactions Browser links
cuff network
rhi network
Reported Interactions
FBrf0217070-1.coIP.WB
Reference
Pane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
cuff 

Cuff

bait

GFP tag

rhi 

Rhi

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovary extract from transgenic line carrying tagged cuff construct.

(Pane et al., 2011)
FBrf0217070-2.coIP.WB
Reference
Pane et al., 2011
Description
physical association
Assay
Collection
Source/Stage
ovary / adult stagefemale
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rhi 

Rhi

bait

GFP tag

cuff 

Cuff

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was ovary extract from transgenic line carrying tagged rhi and cuff constructs.

(Pane et al., 2011)
FBrf0225774-2.coIP.WB
Reference
Le Thomas et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
rhi 

Rhi

bait

BioTAP tag

cuff 

Cuff

prey

GFP tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of transgenic fly line; bait produced from tagged transgenic construct; prey produced from tagged transgenic construct.

(Le Thomas et al., 2014)
FBrf0263998-10.CL.FM
Reference
Ismail et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
cuff 

Cutoff

bait

PH-mCherry tag

rhi 

Rhino

prey

mEGFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

(Ismail et al., 2025)

Source was live S2R+ cells; proteins produced from transfected constructs.

(Ismail et al., 2025)
FBrf0263998-11.CL.FM
Reference
Ismail et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
cuff 

Cutoff

bait

PH-mCherry tag

rhi 

Rhino

prey

mBFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Ismail et al., 2025)

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

(Ismail et al., 2025)
FBrf0263998-13.CL.FM
Reference
Ismail et al., 2025
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2R+
Participants
Corresponds to
Reported as
Role
Note
rhi 

Rhino

bait

PH-mCherry tag

cuff 

Cutoff

prey

mEGFP

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was live S2R+ cells; proteins produced from transfected constructs.

(Ismail et al., 2025)

Bait was fused to a pleckstrin domain with a tag. It removed nuclear proteins to the cytoplasm. Interaction was detected as movement from nuclear proteins to the cytoplasm.

(Ismail et al., 2025)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)