FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
ImpL2, Ilp5
FlyBase ID
FBig0000096810
Interaction Network
Interactions Browser links
ImpL2 network
Ilp5 network
Reported Interactions
FBrf0212648-4.CB
Reference
Sajid et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

ImpL2

bait

secreted from BHK cells into culture medium.

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from transfected construct; prey produced in a yeast expression system.

(Sajid et al., 2011)

Experiment performed with Ilp5 C4 isoform.

(Sajid et al., 2011)
FBrf0212648-5.CL
Reference
Sajid et al., 2011
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

ImpL2

bait

secreted from BHK cells into culture medium.

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from transfected construct; prey produced in a yeast expression system.

(Sajid et al., 2011)

Experiment performed with Ilp5 C4 isoform.

(Sajid et al., 2011)
FBrf0212768-2.FW
Reference
Alic et al., 2011
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

ImpL2

bait

Myc tag

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait secreted into S2 cell culture medium; prey derived from wild-type adult head extract.

(Alic et al., 2011)

Adult head proteins were separated by PAGE, transferred to nitrocellulose and probed with Myc-tagged ImpL2. Ilp5 identified by disappearance of band in Ilp5 mutant extracts.

(Alic et al., 2011)
FBrf0220509-11.coIP.WB
Reference
Okamoto et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp5 

Dilp5

bait

His tag

ImpL2 

Imp-L2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ImpL2 binds most Ilp proteins tested, except for Ilp7.

(Okamoto et al., 2013)

The Sdr and ImpL2 bind Ilp family proteins with different binding affinities.

(Okamoto et al., 2013)

Sdr and ImpL2 do not bind each other.

(Okamoto et al., 2013)

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

(Okamoto et al., 2013)
FBrf0240146-2.XC
Reference
Roed et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

Imp-L2

neutral component
ImpL2 

Imp-L2

neutral component
ImpL2 

Imp-L2

neutral component
Ilp5 

DILP5

neutral component
Ilp5 

DILP5

neutral component
Ilp5 

DILP5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in baculovirus and Sf9 system or a yeast expression system.

(Roed et al., 2018)
FBrf0240146-3.ITC
Reference
Roed et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

Imp-L2

bait
Ilp5 

DILP5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

(Roed et al., 2018)

Kd = 8nM

(Roed et al., 2018)
FBrf0240146-4.SPR
Reference
Roed et al., 2018
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
ImpL2 

Imp-L2

bait
Ilp5 

DILP5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

(Roed et al., 2018)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)