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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000096810
Interaction Network
Interactions Browser links
ImpL2 network
Ilp5 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0212648-4.CB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

ImpL2

bait

secreted from BHK cells into culture medium.

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from transfected construct; prey produced in a yeast expression system.

Experiment performed with Ilp5 C4 isoform.

FBrf0212648-5.CL
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

ImpL2

bait

secreted from BHK cells into culture medium.

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced from transfected construct; prey produced in a yeast expression system.

Experiment performed with Ilp5 C4 isoform.

FBrf0212768-2.FW
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

ImpL2

bait

Myc tag

Ilp5 

Ilp5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait secreted into S2 cell culture medium; prey derived from wild-type adult head extract.

Adult head proteins were separated by PAGE, transferred to nitrocellulose and probed with Myc-tagged ImpL2. Ilp5 identified by disappearance of band in Ilp5 mutant extracts.

FBrf0220509-11.coIP.WB
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ilp5 

Dilp5

bait

His tag

Imp-L2

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

ImpL2 binds most Ilp proteins tested, except for Ilp7.

The Sdr and ImpL2 bind Ilp family proteins with different binding affinities.

Sdr and ImpL2 do not bind each other.

Source was cell extract of HEK293T cell line; bait produced from transfected construct; prey produced from transfected construct.

FBrf0240146-2.XC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Imp-L2

neutral component

Imp-L2

neutral component

Imp-L2

neutral component
Ilp5 

DILP5

neutral component
Ilp5 

DILP5

neutral component
Ilp5 

DILP5

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; protein produced as a recombinant fusion protein in baculovirus and Sf9 system or a yeast expression system.

FBrf0240146-3.ITC
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Imp-L2

bait
Ilp5 

DILP5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

FBrf0240146-4.SPR
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note

Imp-L2

bait
Ilp5 

DILP5

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in baculovirus and Sf9 system; prey produced as a yeast expression system.

External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (4)