FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Abp1, SCAR
FlyBase ID
FBig0000096816
Interaction Network
Interactions Browser links
Abp1 network
SCAR network
Reported Interactions
FBrf0219688-1.PD.WB
Reference
Koch et al., 2012
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abp1 

Abp1

bait

GST tag

Abp1 
Abp1 
SCAR 

SCAR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abp1 
SH3 domain
sufficient binding region
aa 477-531
Abp1 
SH3 domain
mutation disrupting interaction
aa 523

P523A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Koch et al., 2012)

The C-terminal SH3 domain of Abp1 interacts with SCAR, and the N-terminal ADF-H and helical domains of Abp1 interact with microfilaments and liposomes in vitro.

(Koch et al., 2012)
FBrf0219688-2.PD.WB
Reference
Koch et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abp1 

Abp1

bait

GST tag

Abp1 
SCAR 

SCAR

prey
SCAR 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abp1 
SH3 domain
sufficient binding region
aa 477-531
SCAR 
PRD domain
mutation disrupting interaction
aa 457-614
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced from transfected construct in HEK293 cells.

(Koch et al., 2012)

The C-terminal SH3 domain of Abp1 interacts with SCAR, and the N-terminal ADF-H and helical domains of Abp1 interact with microfilaments and liposomes in vitro.

(Koch et al., 2012)
FBrf0219688-3.coIP.WB
Reference
Koch et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Abp1 

Abp1

bait
SCAR 

SCAR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Koch et al., 2012)
FBrf0225064-2.PD.WB
Reference
Koch et al., 2014
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Abp1 

Abp1

bait

GST tag

Abp1 
SCAR 

SCAR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Abp1 
SH3 domain
mutation disrupting interaction
aa 523

P523A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from endogenous gene.

(Koch et al., 2014)

Interactions were brought about by the classical SH3/Pxxp interaction interface.

(Koch et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)