Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey from embryo cDNA expression library).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

The mitochondrial targeting sequence (MTS) of sicily (aa 1-11) is not necessary for its interaction with ND42.

The larger preprotein isoforms of sicily and ND42 preferentially interact with one another (compared to shorter processed isoforms).

Co-translation of sicily increases the amount of ND42 synthesized by in vitro translation.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

The larger preprotein isoforms of sicily and ND42 preferentially interact with one another (compared to shorter processed isoforms).

Co-translation of sicily increases the amount of ND42 synthesized by in vitro translation.

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.