FB2026_03 , released September 17, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
usp, Hr38
FlyBase ID
FBig0000096846
Interaction Network
Interactions Browser links
usp network
Hr38 network
Reported Interactions
FBrf0083464-2.Y2H
Reference
Sutherland et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
usp 

USP

bait

fused to LexA DNA-binding domain

usp 
Hr38 

DHR38

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
usp 
ligand binding domain
sufficient binding region
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Sutherland et al., 1995)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Sutherland et al., 1995)
FBrf0083464-3.PD.WB
Reference
Sutherland et al., 1995
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Hr38 

DHR38

bait

GST tag

usp 

USP

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

(Sutherland et al., 1995)
FBrf0218420-1.FC
Reference
Zoglowek et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hr38 

DHR38

bait

YFP tag, N-terminal fragment

Hr38 
usp 

Usp

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Hr38 
N-terminal region
sufficient binding region
aa 1-551
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was CHO-K1, COS-7 or HeLa cell line; bait produced from transfected construct; prey produced from transfected construct.

(Zoglowek et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)