FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
dsh, kel
FlyBase ID
FBig0000096852
Interaction Network
Interactions Browser links
dsh network
kel network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0221234-5.coIP.WB
Reference
Strutt et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsh 

Dsh

bait

ECFP tag

kel 

Kelch

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

This interaction was not observed in S2 cells, possibly due to poor expression of kel. The interaction was observed upon more robust kel expression in COS-7 cells.

(Strutt et al., 2013)

Source was cell extract of COS-7 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Strutt et al., 2013)
FBrf0259850-1.coIP.WB
Reference
Sun et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
dsh 

dsh

bait

FLAG tag

kel 

kel

prey

mScarlet tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Sun et al., 2024)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)