FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Pp1-87B, PNUTS
FlyBase ID
FBig0000096879
Interaction Network
Interactions Browser links
Pp1-87B network
PNUTS network
Reported Interactions
FBrf0194330-40.Y2H
Reference
Bennett et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pp1-87B 

PP1α87B

bait

fused to GAL4 DNA-binding domain

PNUTS 

PNUTS

prey

fused to VP16 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Bennett et al., 2006)

Two-hybrid system: yeast GAL4-BD/VP16-AD

(Bennett et al., 2006)
FBrf0223236-3.Y2H
Reference
Ciurciu et al., 2013
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pp1-87B 

PP1α87B

bait

fused to GAL4 DNA-binding domain

PNUTS 

dPNUTS

prey

fused to GAL4 activation domain

PNUTS 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PNUTS 
C-terminal region
necessary binding region
aa 631-1135
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Ciurciu et al., 2013)

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Ciurciu et al., 2013)
FBrf0223236-5.coIP.WB
Reference
Ciurciu et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
PNUTS 

dPNUTS

bait

Myc tag

PNUTS 
Pp1-87B 

PP1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
PNUTS 
PP1-binding motif
mutation disrupting interaction
aa 726

W726A

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult nuclear extract of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Ciurciu et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (2)