Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Arfip binds to GTP-bound Rac1, as inferred by its strong interaction with the Rac1 G12V mutant (mimics GTP-locked state) but only weak binding to the Rac1 T17N mutant (mimics the GDP-bound state).

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

Arfip interacts with Rac1 and Arf51F, but not Cdc42.