In vitro interaction; bait produced as a recombinant fusion protein in bacterial system; prey produced in bacterial system (prey from embryo cDNA expression library).

PI31 protein labeled by protein kinase A via the HMK tag, then used to screen a cDNA expression library.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

The PARP domain of tankyrase is required for ADP-ribosylation of PI31 in vitro.

ADP-ribosylation of PI31 by tankyrase is inhibited by the small molecule XAV939.

ADP-ribosylation of PI31 can be detected in vivo.

Transfer of biotin labeled NAD+ substrate to PI31 detected using Streptavidin-HRP and PAGE separation of proteins by molecular weight.