FlyBase Gene Report: Dmel\Tnks

General Information

Symbol

Dmel\Tnks

Species

D. melanogaster

Name

Tankyrase

Annotation Symbol

CG4719

Feature Type

protein_coding_gene

FlyBase ID

FBgn0027508

Gene Model Status

Current

Stock Availability

14 publicly available

Enzyme Name (EC)

NAD(+) ADP-ribosyltransferase (2.4.2.30)

Gene Summary

Tankyrase (Tnks) encodes an ADP-D-ribose polymerase that adds ADP-D-ribose to proteins to form poly-ADP-D-ribose chains (PARylation). Tnks regulates Wnt signalling by mediating the poly-ADP-ribosylation of the Axn protein, resulting in its degradation. [Date last reviewed: 2025-01-23] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Interactive Fly

Also Known As

BcDNA:LD22548 , DTNKS

Key Links

Genomic Location

Cytogenetic map

96E7-96E8

Sequence location

3R:25,653,091..25,661,286 [-]

Recombination map

3-88

RefSeq locus

NT_033777 REGION:25653091..25661286

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (13 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables NAD+ poly-ADP-ribosyltransferase activity

inferred from direct assay

(Croy et al., 2016, Cho-Park and Steller, 2013)

inferred from genetic interaction with FLYBASE:Axn; FB:FBgn0026597

(Tacchelly-Benites et al., 2018, Yang et al., 2016)

inferred from mutant phenotype

(Wang et al., 2019, Wang et al., 2016)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables NAD+ poly-ADP-ribosyltransferase activity

inferred from electronic annotation with InterPro:IPR012317

(InterPro Project Members, 2004-)

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in positive regulation of canonical Wnt signaling pathway

inferred from direct assay

(Croy et al., 2016)

inferred from mutant phenotype

(Wang et al., 2019, Wang et al., 2016, Yang et al., 2016)

inferred from genetic interaction with FLYBASE:Axn; FB:FBgn0026597

(Tacchelly-Benites et al., 2018, Feng et al., 2014)

involved_in positive regulation of JNK cascade

inferred from direct assay

(Wang et al., 2022)

involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process

inferred from direct assay

(Cho-Park and Steller, 2013)

inferred from mutant phenotype

(Wang et al., 2019)

involved_in positive regulation of protein catabolic process

inferred from mutant phenotype

(Feng et al., 2014)

involved_in positive regulation of ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Wang et al., 2016)

NOT involved_in larval chitin-based cuticle development

inferred from mutant phenotype

(Croy et al., 2016)

NOT involved_in segment polarity determination

inferred from mutant phenotype

(Croy et al., 2016)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of canonical Wnt signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

involved_in positive regulation of telomere capping

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

involved_in protein localization to chromosome, telomeric region

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

is_active_in cytosol

inferred from direct assay

(Croy et al., 2016)

located_in nucleus

inferred from direct assay

(Croy et al., 2016)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000652929

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

ADP-RIBOSYLTRANSFERASES

Pathway (FlyBase)

POSITIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY

Protein Family (UniProt)

Belongs to the ARTD/PARP family. (Q9VBP3)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

NAD+ poly-ADP-ribosyltransferase activity

NAD(+) + (ADP-D-ribosyl)n-acceptor = nicotinamide + (ADP-D- ribosyl)n+1-acceptor + H(+) (2.4.2.30)

Summaries

Gene Snapshot

Pathway (FlyBase)

POSITIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY -: Positive regulators of Wnt-TCF (canonical Wnt) signaling up-regulate the pathway, resulting in the enhancement of transcriptional regulation mediated by β-catenin (arm).

Gene Group (FlyBase)

ADP-RIBOSYLTRANSFERASES -: ADP-ribosyltransferases catalyze the addition of single or multiple ADP-ribose moieties from NAD+ donor to the substrates forming mono ADP-ribosylation or poly ADP-ribosylation. (Adapted from PMID:25938242).

Protein Function (UniProtKB)

Stimulates proteasome activity, probably by ADP-ribosylation of PI31 (PubMed:23622245). Modulates 26S proteasome assembly (PubMed:23622245).

(UniProt, Q9VBP3)

Summary (Interactive Fly)

a positive regulator in the JNK signaling pathway - initiates degradation-independent ubiquitination on two lysine residues of JNK to promote its kinase activity and in vivo functions - Proteolysis of TNKS substrates is mediated through their ubiquitination by the poly-ADP-ribose (pADPr)-dependent RING-domain E3 ubiquitin ligase RNF146/Iduna - mediates proteolysis of Axin - downregulation of tankyrase reduces TDP-43 accumulation in the cytoplasm and potently mitigates neurodegeneration - Wingless pathway activation is promoted by a critical threshold of Axin maintained by the tumor suppressor APC and the ADP-ribose polymerase Tankyrase

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Tnks for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VBP3)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.44

Stop-codon suppression (UGA) postulated; FBrf0216885.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Tnks-RA

FBtr0084890

NM_143153

5486

1181

Tnks-RB

FBtr0330383

NM_001276034

5486

1520

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Tnks-PA

FBpp0084264

127.9

1181

6.97

FBpp0303409

165.6

1520

7.58

A0A0B4KI19

NP_001262963

AGB96343

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Interacts (via ANK repeats) with PI31.

(UniProt, Q9VBP3)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Tnks using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Tnks_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.53

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

is_active_in cytosol

inferred from direct assay

(Croy et al., 2016)

located_in nucleus

inferred from direct assay

(Croy et al., 2016)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Tnks in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Tnks

View exonic expression by tissue for Dmel\Tnks

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027508

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43095/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027508

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027508

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027508

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4719

Flygut - An atlas of the Drosophila adult midgut

FBgn0027508

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 10 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Tnks

Transgenic constructs containing regulatory region of Tnks

Aberrations (Deficiencies and Duplications) ( 1 )

Inferred from experimentation ( 1 )

Gene partially disrupted in

Df(3R)RASSF8⁶

(Langton et al., 2009)

Inferred from location ( 1 )

Df(3R)BSC321

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Tnks^GD11407

viable, with Scer\GAL4^Mef2.PR

Tnks^GD11407

viable, with Scer\GAL4^pnr-MD237

Tnks^GD11407

viable, with Scer\GAL4^tin.CΔ4

Tnks^GD11407

Sterility

Allele

fertile

Tnks^m250

Other Phenotypes

Allele

increased cell number | adult stage, with Scer\GAL4^NP0001

increased cell number | adult stage | cell non-autonomous | somatic clone

Tnks¹⁹

increased cell number | adult stage | progressive (with Tnks¹⁹)

Tnks⁵⁰³

increased cell number | adult stage | progressive (with Tnks⁵⁰³)

Tnks¹⁹

increased cell number | adult stage | somatic clone

Tnks¹⁹

increased occurrence of cell division | adult stage, with Scer\GAL4^NP0001

increased occurrence of cell division | adult stage (with Tnks¹⁹)

Tnks⁵⁰³

increased occurrence of cell division | adult stage (with Tnks⁵⁰³)

Tnks¹⁹

short lived | nutrition conditional (with Tnks¹⁹)

Tnks⁵⁰³

short lived | nutrition conditional (with Tnks⁵⁰³)

Tnks¹⁹

Phenotype manifest in

Allele

adult midgut, with Scer\GAL4^NP0001

adult midgut (with Tnks¹⁹)

Tnks⁵⁰³

adult midgut (with Tnks⁵⁰³)

Tnks¹⁹

adult midgut | cell non-autonomous | somatic clone

Tnks¹⁹

adult midgut | somatic clone

Tnks¹⁹

enteroblast | adult stage, with Scer\GAL4^NP0001

enteroblast | adult stage (with Tnks¹⁹)

Tnks⁵⁰³

enteroblast | adult stage (with Tnks⁵⁰³)

Tnks¹⁹

intestinal stem cell | adult stage (with Tnks¹⁹)

Tnks⁵⁰³

intestinal stem cell | adult stage (with Tnks⁵⁰³)

Tnks¹⁹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (113)

Hsap\TNKS

13 of 14

Yes

Hsap\TNKS2

12 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Hsap\ANKRD60

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ANKRD2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ANKRD20A1

1 of 14

Yes

Hsap\ANKRD20A2P

1 of 14

Yes

Hsap\ANKRD20A3P

1 of 14

Yes

Hsap\ANKRD20A4P

1 of 14

Yes

Hsap\ANKRD20A8P

1 of 14

Yes

Hsap\ANKRD26

1 of 14

Yes

Hsap\ANKRD27

1 of 14

Hsap\ANKRD29

1 of 14

Hsap\ANKRD30A

1 of 14

Yes

Hsap\ANKRD30B

1 of 14

Yes

Hsap\ANKRD31

1 of 14

Yes

Hsap\ANKRD35

1 of 14

Yes

Hsap\ANKRD36B

1 of 14

Yes

Hsap\ANKRD36C

1 of 14

Yes

Hsap\ANKRD36

1 of 14

Yes

Hsap\ANKRD42

1 of 14

Hsap\ANKRD45

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ASB5

1 of 14

Yes

Hsap\ASB7

1 of 14

Hsap\ASB8

1 of 14

Yes

Hsap\ASB9

1 of 14

Yes

Hsap\ASB11

1 of 14

Yes

Hsap\ASB13

1 of 14

Yes

Hsap\ASB15

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\CCDC144CP

1 of 14

Yes

Hsap\CDKN2A

1 of 14

Yes

Hsap\CDKN2B

1 of 14

Yes

Hsap\CDKN2C

1 of 14

Yes

Hsap\CDKN2D

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\INVS

1 of 14

Yes

Hsap\IQANK1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\PARP11

1 of 14

Yes

Hsap\PARP12

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

3922

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TONSL

1 of 14

Hsap\USH1G

1 of 14

Hsap\ZC3HAV1

1 of 14

Yes

Hsap\ZDHHC13

1 of 14

Hsap\ZDHHC17

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (86)

Rnor\Tnks

13 of 14

Yes

Rnor\Tnks2

11 of 14

Yes

Rnor\Ankrd42

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ankfy1

1 of 14

Rnor\Ankk1

1 of 14

Yes

Rnor\Ankmy2

1 of 14

Rnor\Ankrd1

1 of 14

Yes

Rnor\Ankrd2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ankrd24

1 of 14

Yes

Rnor\Ankrd26

1 of 14

Yes

Rnor\Ankrd27

1 of 14

Yes

Rnor\Ankrd31

1 of 14

Yes

Rnor\Ankrd35

1 of 14

Yes

Rnor\Ankrd46

1 of 14

Yes

Rnor\Ankrd50

1 of 14

Rnor\Ankrd53

1 of 14

Yes

Rnor\Ankrd55

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Asb5

1 of 14

Yes

Rnor\Asb7

1 of 14

Rnor\Asb8

1 of 14

Yes

Rnor\Asb9

1 of 14

Yes

Rnor\Asb11

1 of 14

Yes

Rnor\Asb13

1 of 14

Yes

Rnor\Aspg

1 of 14

Rnor\Bard1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Parp11

1 of 14

Yes

Rnor\Parp12

1 of 14

Yes

Rnor\Parp14

1 of 14

Rnor\Pidd1

1 of 14

Rnor\Poteh

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tanc2

1 of 14

Rnor\Tiparp

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Zdhhc13

1 of 14

Rnor\Zdhhc17

1 of 14

Mus musculus (laboratory mouse) (89)

Mmus\Tnks

13 of 14

Yes

Mmus\Tnks2

12 of 14

Yes

Mmus\Ankrd42

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ankrd2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ankrd26

1 of 14

Yes

Mmus\Ankrd27

1 of 14

Mmus\Ankrd29

1 of 14

Mmus\Ankrd31

1 of 14

Yes

Mmus\Ankrd35

1 of 14

Yes

Mmus\Ankrd45

1 of 14

Yes

Mmus\Ankrd46

1 of 14

Yes

Mmus\Ankrd55

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Asb5

1 of 14

Yes

Mmus\Asb7

1 of 14

Mmus\Asb8

1 of 14

Yes

Mmus\Asb9

1 of 14

Yes

Mmus\Asb11

1 of 14

Yes

Mmus\Asb13

1 of 14

Yes

Mmus\Aspg

1 of 14

Mmus\Bard1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Iqank1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Parp11

1 of 14

Yes

Mmus\Parp12

1 of 14

Yes

Mmus\Parp14

1 of 14

Mmus\Pidd1

1 of 14

Mmus\Poteg

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Zdhhc13

1 of 14

Mmus\Zdhhc17

1 of 14

Xenopus tropicalis (Western clawed frog) (74)

Xtro\tnks

10 of 13

Yes

Xtro\tnks2

9 of 13

Yes

Xtro\ankrd17

2 of 13

Xtro\ankrd28

2 of 13

Yes

Xtro\ankrd42

2 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ankrd6

1 of 13

Yes

Xtro\ankrd7

1 of 13

Yes

Xtro\ankrd10

1 of 13

Yes

Xtro\ankrd11

1 of 13

Xtro\ankrd12

1 of 13

Xtro\ankrd27

1 of 13

Yes

Xtro\ankrd34a

1 of 13

Yes

Xtro\ankrd50

1 of 13

Xtro\ankrd52

1 of 13

Xtro\ankrd53

1 of 13

Yes

Xtro\ankrd55

1 of 13

Xtro\ankrd60

1 of 13

Yes

Xtro\ankrd65

1 of 13

Xtro\asb2

1 of 13

Xtro\asb9

1 of 13

Yes

Xtro\asb14

1 of 13

Xtro\aspg

1 of 13

Xtro\bard1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ehmt2

1 of 13

Xtro\fem1c

1 of 13

Xtro\gamt

1 of 13

Yes

Xtro\ibtk

1 of 13

Xtro\invs

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100490559

1 of 13

Yes

Xtro\LOC100494827

1 of 13

Yes

Xtro\LOC100494987

1 of 13

Yes

Xtro\LOC100497775

1 of 13

Xtro\LOC100498424

1 of 13

Xtro\LOC101732240

1 of 13

Yes

Xtro\LOC101732478

1 of 13

Xtro\LOC101732564

1 of 13

Yes

Xtro\LOC101732603

1 of 13

Yes

Xtro\LOC101735265

1 of 13

Yes

Xtro\LOC108648817

1 of 13

Yes

Xtro\LOC108648819

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\ppp1r12a

1 of 13

Xtro\ppp1r16a

1 of 13

Xtro\psmd10

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (93)

Drer\tnksa

13 of 14

Yes

Drer\tnksb

12 of 14

Yes

3 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ankrd2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ankrd39

1 of 14

Drer\ankrd44

1 of 14

Drer\ankrd45

1 of 14

Yes

Drer\ankrd55

1 of 14

Drer\ankrd67

1 of 14

Yes

Drer\anks4b

1 of 14

Drer\asb5a

1 of 14

Yes

Drer\asb5b

1 of 14

Yes

Drer\asb11

1 of 14

Yes

Drer\asb13a.1

1 of 14

Yes

Drer\asb13a.2

1 of 14

Yes

Drer\asb13b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\LOC100332428

1 of 14

Yes

Drer\LOC100535451

1 of 14

Drer\LOC103910991

1 of 14

Yes

Drer\LOC108182171

1 of 14

Drer\LOC110438850

1 of 14

Yes

Drer\myo16

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\parp12a

1 of 14

Yes

Drer\parp12b

1 of 14

Yes

Drer\parp14rs1

1 of 14

Drer\parp14rs2.1

1 of 14

Yes

Drer\parp14rs3

1 of 14

Yes

Drer\parp14rs4

1 of 14

Yes

Drer\parp142.2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\si:cabz01054396.2

1 of 14

Yes

Drer\si:ch73-252i11.1

1 of 14

Yes

Drer\si:ch211-223a10.1

1 of 14

Drer\si:ch211-244b2.4

1 of 14

Yes

Drer\si:ch211-272n13.3

1 of 14

Yes

Drer\si:ch1073-398c20.3

1 of 14

Yes

Drer\tiparp

1 of 14

Yes

Drer\tonsl

1 of 14

Drer\trpn1

1 of 14

Drer\uacab

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Caenorhabditis elegans (Nematode, roundworm) (30)

Cele\trp-4

3 of 14

Yes

Cele\arcp-1

1 of 14

Cele\brd-1

1 of 14

Yes

Cele\C01G10.1

1 of 14

Yes

Cele\C06G3.4

1 of 14

Yes

Cele\C18H2.4

1 of 14

Yes

Cele\C18H2.5

1 of 14

Yes

Cele\D1065.3

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\F36D3.5

1 of 14

Yes

Cele\F37A4.4

1 of 14

Yes

Cele\F56D5.9

1 of 14

Yes

Cele\F56H1.3

1 of 14

Yes

Cele\fem-1

1 of 14

Cele\gfi-2

1 of 14

Cele\K09D9.11

1 of 14

Yes

Cele\kdin-1

1 of 14

Cele\lido-18

1 of 14

Yes

Cele\mel-11

1 of 14

Cele\sel-15

1 of 14

Cele\T08G3.7

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (37)

Agam\AgaP_AGAP001947

11 of 12

Yes

Agam\AgaP_AGAP003519

2 of 12

Agam\AgaP_AGAP004215

2 of 12

Agam\AgaP_AGAP008471

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Agam\AgaP_AGAP011724

2 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (32)

Atha\XBAT31

3 of 13

Yes

Atha\AT2G03430

2 of 13

Atha\AT3G24530

2 of 13

Yes

Atha\XBAT32

2 of 13

Yes

Atha\XBAT33

2 of 13

Yes

Atha\AKR2

1 of 13

Yes

Atha\AKR2B

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT4G03490

1 of 13

Atha\AT4G03500

1 of 13

Atha\AT4G19150

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (11)

Scer\NAS6

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\RMT2

1 of 13

Yes

Scer\YGL242C

1 of 13

Yes

Schizosaccharomyces pombe (Fission yeast) (4)

Spom\akr1

1 of 12

Yes

Spom\res1

1 of 12

Yes

Spom\res2

1 of 12

Yes

Spom\SPAC105.02c

1 of 12

Yes

Escherichia coli (enterobacterium) (2)

Ecol\ansA

1 of 11

Yes

Ecol\ansB

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Tnks. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (30)

nompC

4 of 13

Ank2

3 of 13

Ank

3 of 13

3 of 13

3 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Git

1 of 13

Hip14

1 of 13

Ilk

1 of 13

Mbs

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Rel

1 of 13

Sans

1 of 13

wtrw

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Tnks^NIG.4719R

ameliorates amyotrophic lateral sclerosis type 10

modeled by Hsap\TARDBP^UAS.cEa

(McGurk et al., 2018)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

13 of 14

12 of 14

ANKRD44; ankyrin repeat domain 44

3 of 14

620861

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Tnks - Apc2

anti tag coimmunoprecipitation, western blot, two hybrid, pull down

(Croy et al., 2016)

Tnks - Axn

anti tag coimmunoprecipitation, anti tag western blot

(Feng et al., 2014)

Tnks - PI31

enzymatic study, molecular weight estimation by staining, anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, anti tag western blot, far western blotting, autoradiography

(Cho-Park and Steller, 2013)

Tnks - bsk

anti tag coimmunoprecipitation, anti tag western blot

(Li et al., 2018)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Tnks

suppressible

Axn

(Wang et al., 2016)

Tnks

suppressible

upd3

(Wang et al., 2016)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Axn

suppressible

Tnks

(Feng et al., 2014)

Axn

enhanceable

Tnks

(Feng et al., 2014)

enhanceable

Tnks

(Bronstein et al., 2010)

Chi

suppressible

Tnks

(Bronstein et al., 2010)

Prosβ2|Prosβ6

enhanceable

Tnks

(Cho-Park and Steller, 2013)

Ssdp

suppressible

Tnks

(Bronstein et al., 2010)

External Data

Subunit Structure (UniProtKB)

Interacts (via ANK repeats) with PI31.

(UniProt, Q9VBP3 )

Linkouts

BioGRID - A database of protein and genetic interactions.

68007

DroID - A comprehensive database of gene and protein interactions.

FBgn0027508

MIST (genetic) - An integrated Molecular Interaction Database

43095

MIST (protein-protein) - An integrated Molecular Interaction Database

43095

Pathways

Signaling Pathways (FlyBase)

POSITIVE REGULATORS OF WNT-TCF SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VBP3

Class of Gene

gene_with_stop_codon_read_through

(Jungreis, 2011.11.18)

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-88

Cytogenetic map

96E7-96E8

Sequence location

3R:25,653,091..25,661,286 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

96E7-96E8

Limits computationally determined from genome sequence between P{EP}EP3354 and P{PZ}l(3)rQ197^rQ197

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-88

(FlyBase, 2016)

3-86.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

71375

w¹¹¹⁸; P{VT047418-p65.AD}attP40

72911

w¹¹¹⁸; P{VT047418-GAL4.DBD}attP2

22129

w¹¹¹⁸; P{EPg}Tnks^HP37069

42325

y¹ w^*; Mi{MIC}Tnks^MI05449

17132

w¹¹¹⁸; P{EP}Tnks^EP3476/TM6B, Tb¹

60229

y¹ w^*; Mi{PT-GFSTF.1}Tnks^{MI05449-GFSTF.1}

Korea

13542

P{VT047418-GAL4}attP2

17560

P{VT047418-lexA::GADfl}attP40

4719R-3

v106238

v21930

w¹¹¹⁸; P{GD11407}v21930

v21932

w¹¹¹⁸; P{GD11407}v21932

More stocks available...

Genomic Clones (24)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (139)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD22548

Other clones

FI03751

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0027508

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0027508

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Wang et al., 2016, Cho-Park and Steller, 2013)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Relationship to Other Genes

Source for database merge of

Source for merge of: BcDNA:LD22548 CG17487

(FlyBase, 1996-)

Additional comments

Source for merge of BcDNA:LD22548 CG17487 was sequence comparison ( date:001216 ).

(FlyBase, 1996-)

Source for identity of BcDNA:LD22548 anon-WO0100849 was sequence comparison ( date:010129 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: tankyrase CG4719

(Sbodio et al., 2002)

Source for identity of: BcDNA:LD22548 anon-WO0100849

(FlyBase, 1996-)

Source for identity of: Tnks tankyrase

(Cho-Park and Steller, 2013)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (14)

Reported As

Symbol Synonym

BcDNA:LD22548

(Bronstein et al., 2010, Giot et al., 2003, Lai et al., 2003)

CG17487

CG4719

(Aguilera-Gomez et al., 2016, Feng et al., 2014, Cho-Park and Steller, 2013, Bronstein et al., 2010, Keleman et al., 2009.8.5)

DTNKS

(Feng et al., 2018, Feng et al., 2014)

Dm.pARTb

(Otto et al., 2005)

TNKS

(Gultekin and Steller, 2019, Croy et al., 2016)

Tankyrase

(Wang et al., 2016)

TkrsIR

(Liguori et al., 2021)

Tnks

(Gan et al., 2021, Layalle et al., 2021, Wang et al., 2019, Li et al., 2018, McGurk et al., 2018, Perochon et al., 2018, Tian et al., 2018, Transgenic RNAi Project members, 2017-, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Yang et al., 2016, Gene Disruption Project members, 2015-)

dARTD5

(Aguilera-Gomez et al., 2016)

dTANK

(Aguilera-Gomez et al., 2016)

dTNKS

(Cho-Park and Steller, 2013)

tankyrase

(Japanese National Institute of Genetics, 2012.5.21, Jungreis, 2011.11.18, Langton et al., 2009, Buszczak et al., 2007)

Name Synonyms

Tankyrase

(Tian et al., 2018, Wang et al., 2016, Wang et al., 2016, Yang et al., 2016, Kwon et al., 2008)

dTankyrase

(Aguilera-Gomez et al., 2016)

tankyrase

(Feng et al., 2018, McGurk et al., 2018, Feng et al., 2014, Arancio et al., 2010, Langton et al., 2009)

Secondary FlyBase IDs

FBgn0039351

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 45 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

43095

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VBP3

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VBP3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

A0A0B4KI19

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VBP3

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0027508

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

43095/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0027508

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0027508

FlyMine - An integrated database for Drosophila genomics

FBgn0027508

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG4719

MARRVEL_MODEL - MARRVEL (model organism gene)

43095

Linkouts

BioGRID - A database of protein and genetic interactions.

68007

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0027508

DroID - A comprehensive database of gene and protein interactions.

FBgn0027508

DRSC - Results frm RNAi screens

FBgn0027508

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Tnks_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0027508

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0027508

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG4719

Flygut - An atlas of the Drosophila adult midgut

FBgn0027508

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0027508

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC004859

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (genetic) - An integrated Molecular Interaction Database

43095

MIST (protein-protein) - An integrated Molecular Interaction Database

43095

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9VBP3

References (66)

The following external sites may use different assemblies or annotations than FlyBase.

-0.53

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