FlyBase Gene Report: Dmel\Ilk

General Information

Symbol

Dmel\Ilk

Species

D. melanogaster

Name

Integrin linked kinase

Annotation Symbol

CG10504

Feature Type

protein_coding_gene

FlyBase ID

FBgn0028427

Gene Model Status

Current

Stock Availability

16 publicly available

Gene Summary

Integrin linked kinase (Ilk) encodes a pseudokinase that, together with the products of stck and parvin, mediates part of the adhesive functions of integrins. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

l(3)78Ca

Key Links

Genomic Location

Cytogenetic map

78C2-78C2

Sequence location

3L:21,216,901..21,219,240 [+]

Recombination map

3-47

RefSeq locus

NT_037436 REGION:21216901..21219240

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (12 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables ATP binding

inferred from electronic annotation with InterPro:IPR000719

(InterPro Project Members, 2004-)

enables protein kinase activity

inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR001245

(InterPro Project Members, 2004-)

enables protein-macromolecule adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

enables signaling receptor binding

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

NOT enables protein kinase activity

inferred from key residues

(Anamika et al., 2009)

Biological Process (6 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in apposition of dorsal and ventral imaginal disc-derived wing surfaces

inferred from mutant phenotype

(Zervas et al., 2001)

involved_in cell-matrix adhesion

inferred from mutant phenotype

(Zervas et al., 2001)

involved_in somatic muscle attachment to chitin-based cuticle

inferred from mutant phenotype

(Liu et al., 2013, Zervas et al., 2001)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cell differentiation

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

involved_in cell-matrix adhesion

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

involved_in integrin-mediated signaling pathway

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

involved_in substrate adhesion-dependent cell spreading

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN002892787

(GO Reference Genome Project, 2011-)

is_active_in focal adhesion

inferred from biological aspect of ancestor with PANTHER:PTN001510364

(GO Reference Genome Project, 2011-)

located_in focal adhesion

traceable author statement

(Hynes and Zhao, 2000)

Gene Group (FlyBase)

UNCLASSIFIED PSEUDOKINASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Integrin linked kinase (Ilk) encodes a pseudokinase that, together with the products of stck and parvin, mediates part of the adhesive functions of integrins. [Date last reviewed: 2019-03-07]

Gene Group (FlyBase)

UNCLASSIFIED PSEUDOKINASES -: This group comprises pseudokinases that do not classify under other groups in FlyBase.

Summary (Interactive Fly)

s/t kinase with ankyrin repeats - required for integrin-mediated adhesion - links the cytoskeleton via integrins to the plasma membrane

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Ilk for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9V400)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Ilk-RA

FBtr0078305

NM_080262

1806

448

Ilk-RB

FBtr0307160

NM_001259931

671

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Ilk-PA

FBpp0077962

50.7

448

7.49

FBpp0297989

3.7

4.19

E3CTS5

NP_001246860

AFH04531

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Ilk using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-1.39

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 5

organism | faint

(Zervas et al., 2001)

embryonic stage 6 -- 13

mesoderm

(Zervas et al., 2001)

ectoderm | restricted

Comment: part nearest mesoderm

(Zervas et al., 2001)

embryonic stage 11

hindgut proper primordium

(Zervas et al., 2001)

stomodeal invagination

(Zervas et al., 2001)

posterior midgut primordium

(Zervas et al., 2001)

embryonic stage 11 -- 12

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

trunk visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

embryonic stage 13

endoderm

(Zervas et al., 2001)

epidermis

(Zervas et al., 2001)

embryonic stage 13 -- 16

embryonic visceral muscle cell

(Zervas et al., 2001)

embryonic somatic muscle cell

(Zervas et al., 2001)

dorsal pharyngeal muscle cell

(Fisher et al., 2012)

embryonic/larval garland cell

(Fisher et al., 2012)

larval muscle system

(Fisher et al., 2012)

larval somatic muscle cell

(Fisher et al., 2012)

larval visceral muscle cell

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in focal adhesion

inferred from direct assay

(Delon and Brown, 2009)

Expression Deduced from Reporters

Reporter: P{Ilk.mGFP6}

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 16 -- 17

muscle attachment site

(Loer et al., 2008)

adult stage

muscle attachment site

(Krzemien et al., 2012)

Reporter: P{PTT-GB}Ilk^ZCL3111

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

lch5 ligament attachment cell | vicinity of

(Halachmi et al., 2016)

lch5 cap attachment cell | vicinity of

(Halachmi et al., 2016)

scolopidial dendritic cap cell | vicinity of

(Halachmi et al., 2016)

scolopidial ligament cell | vicinity of

(Halachmi et al., 2016)

adult stage

adult brain cell body glial cell

(DeSalvo et al., 2014)

adult brain surface glial cell

(DeSalvo et al., 2014)

adult stage | female

peritoneal sheath muscle cell

• costamere

(Hudson et al., 2008)

ovarian sheath muscle cell

• costamere

(Hudson et al., 2008)

common oviduct circular muscle cell

• costamere

(Hudson et al., 2008)

oogenesis & adult stage | female

follicle cell

• nucleus

(Cooley et al., 2003.5)

follicle cell

• cytoplasm

(Cooley et al., 2003.5)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Ilk in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Ilk

View exonic expression by tissue for Dmel\Ilk

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028427

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53573/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028427

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028427

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028427

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10504

Flygut - An atlas of the Drosophila adult midgut

FBgn0028427

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Ilk

Stages(s) 1-3
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 5 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 34 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Ilk

Transgenic constructs containing regulatory region of Ilk

Aberrations (Deficiencies and Duplications) ( 16 )

Inferred from experimentation ( 16 )

Gene disrupted in

Gene not disrupted in

Inferred from location ( 3 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^e22c

Ilk^GD6996

lethal, with Scer\GAL4^Mef2.PR

Ilk^GD6996

lethal (with Ilk¹)

Ilk²

lethal (with Ilk²)

Ilk¹

lethal - all die before end of first instar larval stage, with Scer\GAL4^how-24B

Ilk^GL00288

lethal - all die before end of first instar larval stage, with Scer\GAL4^Mef2.PR

Ilk^GL00288

lethal - all die during embryonic stage, with Scer\GAL4^Mef2.PR

Ilk^GD6996

lethal - all die during embryonic stage | recessive

Ilk¹

partially lethal, with Scer\GAL4^tin.CΔ4

Ilk^GD6996

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Ilk^GD6996

viable, with Scer\GAL4^pnr-MD237

Ilk^GD6996

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Ilk^HMS04509

Other Phenotypes

Allele

abnormal cell shape | third instar larval stage, with Scer\GAL4^pnr-MD237

Ilk^GD6996

abnormal size | adult stage | somatic clone

Ilk¹

decreased cell number | adult stage | somatic clone

Ilk¹

increased cell size | third instar larval stage, with Scer\GAL4^pnr-MD237

Ilk^GD6996

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Ilk^GD6996

some die during embryonic stage | recessive

Ilk¹

visible | recessive | somatic clone

Ilk¹

wild-type

wild-type, with Scer\GAL4^48Y

wild-type, with Scer\GAL4^69B

wild-type, with Scer\GAL4^arm.PS

wild-type, with Scer\GAL4^en-e16E

wild-type, with Scer\GAL4^how-24B

wild-type, with Scer\GAL4^twi.PG

Phenotype manifest in

Allele

adult posterior midgut epithelium | somatic clone

Ilk¹

embryonic/larval epidermis | third instar larval stage, with Scer\GAL4^pnr-MD237

Ilk^GD6996

embryonic/larval hypodermal muscle cell | third instar larval stage, with Scer\GAL4^pnr-MD237

Ilk^GD6996

larval somatic muscle cell

Ilk¹

larval somatic muscle cell, with Scer\GAL4^Mef2.PR

Ilk^GD6996

larval somatic muscle cell (with Df(3L)Pc-14d)

Ilk¹

visceral muscle fiber | third instar larval stage | heat sensitive, with Scer\GAL4^Mef2.PR, Scer\GAL80^ts.αTub84B

Ilk^GL00288

wing | somatic clone

Ilk¹

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (25)

Hsap\ILK

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\ANKRD20A2P

1 of 14

Yes

Hsap\ANKRD20A3P

1 of 14

Yes

Hsap\ANKRD20A4P

1 of 14

Yes

Hsap\ANKRD20A8P

1 of 14

Yes

Hsap\ANKRD27

1 of 14

Hsap\ANKRD28

1 of 14

Hsap\ANKRD30B

1 of 14

Yes

Hsap\ANKRD30BL

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\TNNI3K

1 of 14

Hsap\TXK

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (12)

Rnor\Ilk

14 of 14

Yes

Rnor\Acbd6

1 of 14

Rnor\Ank3

1 of 14

Rnor\Ankk1

1 of 14

Yes

Rnor\Ankrd1

1 of 14

Yes

Rnor\Ankrd28

1 of 14

Rnor\Ankrd46

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Tnni3k

1 of 14

Mus musculus (laboratory mouse) (12)

Mmus\Ilk

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ankrd28

1 of 14

Mmus\Ankrd46

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Tnni3k

1 of 14

Xenopus tropicalis (Western clawed frog) (11)

Xtro\ilk

10 of 13

Yes

Xtro\ankrd27

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (14)

Drer\ilk

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ankrd1b

1 of 14

Yes

Drer\ankrd2

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\rai14

1 of 14

Yes

Drer\tnni3k

1 of 14

Drer\zak

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\pat-4

14 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP006573

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (52)

Atha\VIK

3 of 13

Yes

Atha\AT2G31800

2 of 13

Atha\AT2G43850

2 of 13

Atha\AT3G58760

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AKR2B

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Ilk. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (18)

Raf

3 of 13

slpr

2 of 13

Takl1

2 of 13

wnd

2 of 13

Acbp-Ank

1 of 13

Alk

1 of 13

Ank

1 of 13

cdi

1 of 13

CG10809

1 of 13

Git

1 of 13

ksr

1 of 13

LIMK1

1 of 13

Lrrk

1 of 13

Mos

1 of 13

Ror

1 of 13

Tak1

1 of 13

Takl2

1 of 13

Tnks

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

ILK; integrin linked kinase

14 of 14

602366

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Ilk - Hsp60A

experimental knowledge based

(Guruharsha et al., 2011)

Ilk - ics

anti tag coimmunoprecipitation, western blot

(Kadrmas et al., 2004)

Ilk - parvin

two hybrid, experimental knowledge based

(Hu et al., 2017.6.13, Guruharsha et al., 2011)

Ilk - stck

two hybrid, anti tag coimmunoprecipitation, western blot, pull down, peptide massfingerprinting, anti bait coimmunoprecipitation, anti tag western blot

(Pronovost et al., 2013, Yoshigi et al., 2013, Elias et al., 2012, Kadrmas et al., 2004, Clark et al., 2003)

Ilk - wech

anti tag coimmunoprecipitation, anti tag western blot

(Loer et al., 2008)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ics

suppressible

Ilk

(Ojelade et al., 2015)

parvin

suppressible

Ilk|Diap1

(Chountala et al., 2012)

parvin

suppressible

Ilk

(Chountala et al., 2012)

sli

enhanceable

Ilk

(MacMullin and Jacobs, 2006)

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0028427

MIST (protein-protein) - An integrated Molecular Interaction Database

53573

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-47

Cytogenetic map

78C2-78C2

Sequence location

3L:21,216,901..21,219,240 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

78C2-78C2

Limits computationally determined from genome sequence between P{EP}chb^EP3403 and P{PZ}ppl⁰⁰²¹⁷

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

78B2-78B2

(Ali, 2000.1.19)

Experimentally Determined Recombination Data

Location

3-47

(FlyBase, 2016)

3-43.8

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (16)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

6831

w¹¹¹⁸; P{PTT-GB}Ilk^ZCL3111

99924

w^*; P{UAS-Ilk-GFP}2/CyO; P{UAS-Ilk-GFP}3

600248

w^*; P{Ilk.mCherry}2; TM2/TM6B, Tb¹

24825

w¹¹¹⁸; Mi{ET1}Ilk^MB04821/TM6C, Sb¹

600249

w^*; P{Ilk.mGFP6}2A/CyO; Ilk⁵⁴ P{FRT(w^hs)}2A

4861

mwh¹ Ilk¹ red¹ e⁴/TM3, Sb¹

35374

y¹ sc^* v¹ sev²¹; P{TRiP.GL00288}attP2

FlyORF

F003177

M{UAS-Ilk.ORF.3xHA.GW}ZH-86Fb

Kyoto

110951

w^* P{PTT-GB}Ilk^ZCL3111

107914

mwh¹ Ilk¹ red¹ e⁴/TM3, Sb¹

VDRC

v318121

PBac{fTRG00483.sfGFP-TVPTBF}VK00033

v341243

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD00286}attP40/CyO-GFP

More stocks available...

Genomic Clones (25)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (80)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD24671

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0028427

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0028427

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a whole range of mitotic abnormalities, chromosome abnormalities, lagging chromatids, and abnormal spindles are seen.

(Bettencourt-Dias et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Ilk mutations cause embryonic lethality and defects in muscle attachment, and clones of cells lacking Ilk in the adult wing fail to adhere, forming wing blisters.

(Zervas et al., 2001)

Clones in the wing result in wing blisters.

(Zervas et al., 1999)

Relationship to Other Genes

Source for database merge of

Source for merge of: Ilk CG10504

(Bayraktaroglu, 2000.8.7)

Source for merge of: Ilk l(3)78Ca

(Zervas et al., 2001)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (12)

Reported As

Symbol Synonym

CG10504

(Yoshigi et al., 2013, Cammarato et al., 2011, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Quinones-Coello, 2007, Brody et al., 2002, Morrison et al., 2000)

CT29478

(Hynes and Zhao, 2000)

DmILK

(Zervas et al., 1999)

l(3)78Ca

(Russell et al., 1996, Carpenter, 1994)

Name Synonyms

Integrin Linked Kinase

(Elias et al., 2008)

Integrin linked kinase

(Yoshigi et al., 2013, Cammarato et al., 2011, Hudson et al., 2008, Zervas et al., 1999)

Integrin-linked kinase

(Lin et al., 2013, Liu et al., 2013, Zervas et al., 2011, Schotman et al., 2008)

integrin linked kinase

(Knox et al., 2011, Levi et al., 2006)

integrin-linked kinase

(Uchino et al., 2013, Weavers and Skaer, 2013, Yuan et al., 2010, Loer et al., 2008, Wang and Galko, 2008, Lo, 2006, Trainor, 2004, Brower, 2003)

Secondary FlyBase IDs

FBgn0013568
FBgn0037062

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 48 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

53573

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9V400

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9V400

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0028427

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

53573/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0028427

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0028427

FlyMine - An integrated database for Drosophila genomics

FBgn0028427

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10504

MARRVEL_MODEL - MARRVEL (model organism gene)

53573

Linkouts

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0028427

DroID - A comprehensive database of gene and protein interactions.

FBgn0028427

DRSC - Results frm RNAi screens

FBgn0028427

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0028427

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0028427

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10504

Flygut - An atlas of the Drosophila adult midgut

FBgn0028427

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0028427

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC002476

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

53573

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

References (143)

The following external sites may use different assemblies or annotations than FlyBase.

-1.39

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