FlyBase Allele Report: Dmel\Tnks[19]

General Information

Symbol

Dmel\Tnks¹⁹

Species

D. melanogaster

Name

FlyBase ID

FBal0324221

Feature type

allele

Associated gene

Dmel\Tnks

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Wang et al., 2016)

Mutagen

Delta2-3 transposase

(Wang et al., 2016)

Progenitor genotype

P{SUPor-P}KG00687

(Wang et al., 2016)

P{EP}G9172

(Wang et al., 2016)

Cytology

Description

Simultaneous mobilization of P{EP}G9172 and P{SUPor-P}KG00687 results in complete deletion of Tnks (-20 to +9069 with reference to the Tnks transcriptional start site).

(Wang et al., 2016)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut (with Tnks⁵⁰³)

(Wang et al., 2016)

adult midgut | cell non-autonomous | somatic clone

(Wang et al., 2016)

adult midgut | somatic clone

(Wang et al., 2016)

enteroblast | adult stage (with Tnks⁵⁰³)

(Wang et al., 2016)

intestinal stem cell | adult stage (with Tnks⁵⁰³)

(Wang et al., 2016)

Detailed Description

Statement

Reference

Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutants eclose at normal rates and are viable, but adults display a significant increase in mortality when maintained on a sucrose only diet (5% sucrose (v/v) in aqueous solution), as compared to controls.

Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutant adult midguts show a significant increase in the numbers of intestinal stem cells, of intestinal progenitor cells and of mitotic cells, but similar apoptotic levels, as compared to controls; these phenotypes become more severe with age.

Adulthood-generated Tnks¹⁹ homozygous somatic clones in mosaic midguts have a significant increase in cell number as compared to adulthood-generated control somatic clones in wild-type adult midguts but, similarly to wild-type clones, these clones include enteroblasts, enterocytes and enteroendocrine cells.

Generating Tnks¹⁹ homozygous somatic clones in mosaic midguts during adulthood leads to an increase in the number of neighboring control intestinal progenitor cells, heterozygous for Tnks¹⁹; this phenotype is not seen in clones generated during pupation and analyzed at eclosion.

(Wang et al., 2016)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Tnks⁵⁰³/Tnks¹⁹ has increased cell number | adult stage phenotype, suppressible by Axn[+]/Axn^S044230

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has increased cell number | adult stage phenotype, suppressible by upd3^KK110348/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has increased occurrence of cell division | adult stage phenotype, suppressible by Axn[+]/Axn^S044230

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has increased occurrence of cell division | adult stage phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has increased occurrence of cell division | adult stage phenotype, suppressible by upd3^KK110348/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has increased cell number | adult stage phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks¹⁹ has increased cell number | somatic clone | adult stage phenotype, suppressible by Axn^Tag:V5, Axn^S044230/Axn^S044230

(Wang et al., 2016)

Other

Statement

Reference

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has visible | adult stage phenotype

(Wang et al., 2016)

Phenotype Manifest In

Suppressed by

Statement

Reference

Tnks¹⁹ has adult midgut | somatic clone phenotype, suppressible by Axn^Tag:V5, Axn^S044230/Axn^S044230

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has adult midgut phenotype, suppressible by Axn[+]/Axn^S044230

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has enteroblast | adult stage phenotype, suppressible by Axn[+]/Axn^S044230

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has adult midgut phenotype, suppressible by upd3^KK110348/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has intestinal stem cell | adult stage phenotype, suppressible by upd3^KK110348/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has adult midgut phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Wang et al., 2016)

Tnks⁵⁰³/Tnks¹⁹ has intestinal stem cell | adult stage phenotype, suppressible by upd3^GD6811/Scer\GAL4^NP0001

(Wang et al., 2016)

Other

Statement

Reference

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has wing vein phenotype

(Wang et al., 2016)

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has abdominal sternite bristle phenotype

(Wang et al., 2016)

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has wing margin phenotype

(Wang et al., 2016)

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has abdominal sternite phenotype

(Wang et al., 2016)

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has wing margin bristle | ectopic phenotype

(Wang et al., 2016)

Axn^UAS.Tag:V5, Scer\GAL4^71B, Tnks⁵⁰³/Tnks¹⁹ has wing blade phenotype

(Wang et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in the numbers of intestinal progenitor cells and mitotic cells present in Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutant adult midguts is suppressed by Axn^S044230 heterozygosity; the increase in the numbers of intestinal stem cells and mitotic cells observed in Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutant adult midguts are suppressed by expressing either upd3^KK110348 or upd3^GD6811 under the control of Scer\GAL4^{Myo31DF-NP0001}.

Adulthood-generated Tnks¹⁹, Axn^S044230 double homozygous somatic clones expressing the Axn^T:SV5\V5 rescue transgene present a significant decrease in cell number as compared to adulthood-generated Tnks¹⁹ single homozygous somatic clones.

(Wang et al., 2016)

Expression of Axn^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^71B in the Tnks¹⁹/Tnks⁵⁰³ background leads to morphological wing (notches, loss of wing margin, ectopic bristles) and ventral abdomen (loss of sternite and sternite bristles, reduced size of the sternites that remained, expansion of pleura) defects in adults.

(Wang et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Tnks⁵⁰³/Tnks¹⁹ is rescued by Tnks^UAS.Tag:HA/Scer\GAL4^NP0001

(Wang et al., 2016)

Comments

Expressing Tnks^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^{Myo31DF-NP0001} significantly rescues the increase in the numbers of stem cells and mitotic cells observed in the adult midgut of Tnks¹⁹/Tnks⁵⁰³ transheterozygous mutant adults.

(Wang et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Tnks¹⁹

(Wang et al., 2019, Wang et al., 2016, Wang et al., 2016, Wang et al., 2016, Yang et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

Open Close