His2Av, Jabba Physical Interaction report

General Information

Interaction Type

physical association

Interacting Genes

His2Av, Jabba

FlyBase ID

FBig0000096915

Interaction Network

Interactions Browser links

His2Av network
Jabba network

Reported Interactions

FBrf0220040-1.coIP.WB

Reference

Li et al., 2012

Description

physical association

Assay

anti tag coimmunoprecipitation

western blot

Collection

Source/Stage

/ embryonic stage 1 embryonic stage 6 blastoderm stage embryonic stage 5 embryonic stage 3 embryonic cycle 6 embryonic cycle 8 embryonic cycle 5 embryonic cycle 7 embryonic cycle 4 embryonic cycle 3 embryonic stage 4 embryonic stage 2 embryonic cycle 9

Cell line used

Participants

Corresponds to

Reported as

Role

Note

His2Av

H2Av

bait

GFP tag

Jabba

prey

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was lipid droplets from embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2012)

FBrf0234959-38.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, N-terminal fragment

His2Av

H2Av

neutral component

Gaussia luciferase tag, C-terminal fragment

His2Av

H2Av

neutral component

Gaussia luciferase tag, C-terminal fragment

His2Av

H2Av

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Strong interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0234959-49.BiLC.LUC

Reference

Kolkhof et al., 2017

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

His2Av

H2Av

neutral component

Gaussia luciferase tag, N-terminal fragment

His2Av

H2Av

neutral component

Gaussia luciferase tag, N-terminal fragment

His2Av

H2Av

neutral component

Gaussia luciferase tag, N-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Jabba

neutral component

Gaussia luciferase tag, C-terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Strong interaction.

(Kolkhof et al., 2017)

Source was Kc167 cells; proteins produced from transfected constructs.

(Kolkhof et al., 2017)

Cells treated with 400uM oleic acid to induce lipid droplet formation in cells.

(Kolkhof et al., 2017)

FBrf0249777-1.BiLC.LUC

Reference

Stephenson et al., 2021

Description

physical association

Assay

split luciferase complementation

luminiscence technology

Collection

Source/Stage

Cell line used

Kc167

Participants

Corresponds to

Reported as

Role

Note

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Jabba

His2Av

H2Av

unspecified role

Gaussia luciferase tag, N or C terminal fragment

His2Av

H2Av

unspecified role

Gaussia luciferase tag, N or C terminal fragment

His2Av

H2Av

unspecified role

Gaussia luciferase tag, N or C terminal fragment

Experimental entities

Corresponds to

Identifier

Reported

Role

Note

Subregions with role in interaction

Corresponds to

Description

Role

Coordinates

Note

Jabba

interaction domain

sufficient binding region

aa 192-320

Isoform-specific participants

Corresponds to

Description

Role

Note

Comments concerning this interaction

Source was live Kc167 cells; proteins produced from transfected constructs.

(Stephenson et al., 2021)

External Crossreferences and Linkouts ( 1 )

Linkouts

MIST - An integrated Molecular Interaction Database

His2Av,Jabba

References (3)

Report Sections

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